Method for Artificially Raising Corn Aphids Using Live Corn Plants

Abstract

The invention discloses a method for artificially feeding and propagating corn aphids by using live corn plants. The formula soil obtained by mixing loam soil and nutrient soil is selected as the culture substrate of live corn plants, and seeds of aphid-susceptible corn varieties are first sown in the culture substrate. Then cultivate corn plants in the artificial climate box, inoculate the corn aphid source when the corn plants grow to two leaves and one heart, and then continue to cultivate the corn plant growth in the artificial climate box, and carry out the feeding and breeding of corn aphids. The breeding and breeding method of corn aphid obtained is close to the breeding process of corn aphid in the natural state, so as to obtain a large number of corn aphid samples with the same biological characteristics, genetic characteristics and other physiological and biochemical characteristics as the naturally captured corn aphid, so that the supply of insect source samples More stable and more universal, it is of great significance for the detailed and precise biotype research of aphids, the precise positioning and cloning of aphid-resistant genes, molecular marker-assisted breeding and the selection of excellent aphid-resistant maize varieties.

Description

technical field [0001] The invention relates to the technical field of artificial cultivation of corn aphids, in particular to a method for artificially raising and propagating corn aphids by using living corn plants. Background technique [0002] Aphids are extremely common crop pests with a wide host range that can cause damage to almost all major cultivated crops. Aphids belong to the family Aphididae of the order Homoptera and are commonly known as greasy insects or dense insects. There are nearly 5,000 species of aphids in the world, among which the major damage to corn is corn aphid (Rhopalosiphum maidis), followed by sorghum aphid (Melanaphis sacchari). Corn aphids are widely distributed in Northeast, Northwest, North and Central China, and their host plants are diverse, including not only corn, sorghum, rice, wheat and other gramineous crops, but also barnyardgrass, crabgrass, horsetail and other weeds. The adults and nymphs of corn aphids directly damage crops by ...

AI Technical Summary

Problems solved by technology

Corn aphids can have more than 10 generations in northern my country every year, and overwinter as wingless viviparous female aphids on wheat seedlings and grass weeds; winged aphids migrate to spring corn and sorghum fields at the end of April and early May to cause damage; During the reproductive growth period of sowing corn, it spreads to the summer sowing corn field. At this time, the number of insects doubles and enters the stage of serious damage; in the first and middle of October, the corn gradually ages, and the corn aphid produces winged aphids for the last time, and moves into the wheat field to cause damage Therefore, the collection of corn aphids in the field is affected by many factors such as aphid migration, season, weather, etc., making the identification efficiency of aphid resistance low and the results unreliable

For the artificial breeding of aphids, solid medium is often used to raise them in fixed containers at present, such as Chinese patent application CN201710099350.1. Although this breeding method can successfully obtain the reproduction of aphids, the obtained aphid samples are directional induced by the artificial feeding method. There are differences in biological, genetic, and genetic characteristics between aphid samples captured in the natural environment, resulting in large differences between research results and natural control effects, which is not conducive to practical application

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