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Method for extracting semen lepidii neoflavonoid C from semen lepidii and application of lepidium apetalum neoflavonoid C

A technology of Tinglizi and Tinglizi, which is applied in the field of extracting new flavonoid C of Tingli, can solve problems such as no public reports, and achieve the effects of developing medicinal value and commercial value, scientific preparation method, and rich raw materials

Inactive Publication Date: 2018-01-19
HENAN UNIV OF CHINESE MEDICINE
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Problems solved by technology

[0003] With the research on the active ingredients and medicinal value of Tinglizi, it contains many medicinal active ingredients, including new flavonoid C of Tingli, but how to extract it from Fructus Tingli and what effect it has so far No public reports

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  • Method for extracting semen lepidii neoflavonoid C from semen lepidii and application of lepidium apetalum neoflavonoid C
  • Method for extracting semen lepidii neoflavonoid C from semen lepidii and application of lepidium apetalum neoflavonoid C
  • Method for extracting semen lepidii neoflavonoid C from semen lepidii and application of lepidium apetalum neoflavonoid C

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[0014] The specific implementation of the present invention will be described in detail below in combination with specific situations.

[0015] In the specific implementation of the present invention, a new flavonoid C from Tinglizi is extracted from Tinglizi, the molecular structure formula is shown in figure 1 As shown, the preparation method is to stir fry 10kg of Tinglizi at 240°C for 5.5min, after frying, decoct and extract three times with 10 times the weight of Tinglizi, each time for 1.5h, and combine three times The extract is concentrated under reduced pressure at 45°C until it is equivalent to an extract containing 2g / mL of the crude drug (ie 5L of extract). At room temperature, add 25L of ethanol with a volume concentration of 80% to the extract for alcohol precipitation, and let it stand for 1h. Pour out the supernatant, add 25L of ethanol with a volume concentration of 80% for alcohol precipitation again, repeat the operation 4 times, combine the supernatant, con...

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Abstract

The invention relates to a method of extracting semen lepidii neoflavonoid C from semen lepidii and application of the lepidium apetalum neoflavonoid C, effectively extracting semen lepidii neoflavonoid C from semen lepidii and realizing application in preparing estrogen-like effect medicine. The method comprises the following steps: stir-frying the semen lepidii; decocting with water and extracting; concentrating the extract liquid into an extract; performing alcohol precipitation on the extract; loading the supernate on a Dianion HP-20 column; performing gradient elution with water and ethanol; collecting the 40%-ethanol eluent; concentrating and drying; dissolving with water; centrifuging and filtering; treating the filtrate with Toyopearl HW-40 column chromatography; eluting with water; performing alcohol precipitation on the eluent; centrifuging the supernate; filtering; drying the filtrate; treating the filtrate with Sephadex LH-20 column chromatography; eluting with methanol;collecting the fraction, 5ml for each tube; mxing the fractions of 34-41 tubes; separating with semi-preparative HPLC; collecting the fractions of which the retention time tR is 45.0 to 46.0min; concentrating and drying to obtain a compound semen lepidii neoflavonoid C. According to the method provided by the invention, the raw materials are abundant, the preparation method is scientific and easyto operate, the medicinal value and commercial value of the lepidium apetalum are explored, and the economic and social benefits are remarkable.

Description

technical field [0001] The invention relates to medicine, in particular to a method for extracting new flavonoid C from Tinglizi and its application. Background technique [0002] Tinglizi is a commonly used Chinese herbal medicine. It was first recorded in "Shen Nong's Materia Medica", and it was listed as a herbal inferior product. The medicine tastes pungent and bitter, and is very cold in nature. The "Tinglizi" included in the 2015 edition of "Chinese Pharmacopoeia" has a north-south distinction. Among them, the dry mature seeds of the cruciferous plant Lepidium apetalum Willd. .) The dry mature seeds of Webbex Prantl. are commonly called "Nan Ting Li Zi". [0003] With the research on the active ingredients and medicinal value of Tinglizi, it contains many medicinal active ingredients, including new flavonoid C of Tingli, but how to extract it from Fructus Tingli and what effect it has so far No public reports have been seen. Contents of the invention [0004] In v...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/203C07H1/08A61P35/00A61P5/30
Inventor 郑晓珂李孟曾梦楠张靖柯赵璇吕锦锦张志广冯卫生
Owner HENAN UNIV OF CHINESE MEDICINE
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