Urine preservative for preventing free DNAs in urine from being degraded and urine preserving tube
A preservative, urine technology, applied in the field of biological sample preservation, can solve the problem of inability to preserve cell-free DNA and the like
Pending Publication Date: 2018-01-19
王煜
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Problems solved by technology
Even so, low temperature alone cannot effectively preserve cell-free DNA in urine samples for a certain period of time
Method used
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[0016] The principles and features of the present invention are described below in conjunction with examples, which are only used to explain the present invention and are not intended to limit the scope of the present invention.
[0017] Below, Qubit3.0 is used to detect the changes in the content of free DNA in urine. Three urine samples were collected in the hospital, and returned to the laboratory within 1 hour after sampling to separate free DNA and then extracted. A sample of the preservative was used as a reference for comparison.
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The invention relates to a urine preservative for preventing free DNAs in urine from being degraded. The urine preservative is a water solution containing the following substances in percent by weight: 4% to 8% of a nucleic acid inhibitor, 10% to 18% of a preservative, 5% to 10% of a quenching agent and 2% to 5% of a pH buffering agent. The urine preservative can guarantee that urine is preservedfor 7 days at most at the temperature of 4 DEG C, in the period, the free DNAs cannot be degraded obviously, and gDNAs of cast-off cells in the urine cannot be released into the urine obviously to influence the content of the free DNAs in the urine, so that the urine preservative is applicable for polymerase chain reactions and DNA sequencing.
Description
technical field [0001] The invention relates to the field of biological sample preservation, and more particularly relates to a urine preservation agent and a urine preservation tube for preventing the degradation of free DNA in urine. Background technique [0002] The main source of free DNA in normal urine is the degradation of epithelial cells and a small amount of leukocytes shed from organs of the urinary system such as the kidney, bladder, and urethra. When pathological changes occur in the urinary system, the content of free DNA in urine will also increase. Then change. At present, there are more and more studies on cell-free DNA in urine. Urine-based liquid biopsy technology uses cell-free DNA in urine as the detection target. During the detection process, the stability of the initial cell-free DNA is very important. [0003] However, compared with peripheral blood, urine has higher nuclease content, more metabolites, and larger pH range fluctuations, so the biologi...
Claims
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Inventor 王龙李佩王煜
Owner 王煜