Functional marker of rice seed shattering gene qSH1, and application of functional marker

A shattering, SH1-F2 technology, applied in the field of agricultural biology, can solve the problems of difficult shattering, differences in expression parts, affecting the breeding process, etc., and achieve the effects of speeding up the breeding process, improving the breeding efficiency, and improving the breeding efficiency.

Inactive Publication Date: 2018-01-19
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The SNP is located in the RY repeat sequence, which may affect the binding to the ABI3-type transcription factor, resulting in the difference in the expression site of qSH1, resulting in the inability to form a detachment layer at the base of the spikelet of Nipponbare, thus making it difficult to shatter (resistance to shattering)
[0004] At present, there is no functional marker developed for qSH1, and the shattering phenotype can only be judged by the shattering phenotype during the harvest period, which affects the breeding process

Method used

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  • Functional marker of rice seed shattering gene qSH1, and application of functional marker
  • Functional marker of rice seed shattering gene qSH1, and application of functional marker
  • Functional marker of rice seed shattering gene qSH1, and application of functional marker

Examples

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example 1

[0037] Example 1 Detection of 4 Rice Varieties Using Functional Marker SH1

[0038] (1) Genomic DNA was extracted from 4 rice varieties (Nipponbare, which is not easy to shatter, and Dongxiang wild rice, Zengcheng wild rice and Gaozhou wild rice, which are easy to shatter): rice leaves were taken respectively, and rice genomic DNA was obtained by TPS simple method.

[0039] (2) PCR amplification

[0040] The PCR reaction system is a 20 μl reaction system: 2.0 μl of 10×PCR buffer; 0.5 μl of 10 mM dNTPs; 0.5 μl of 10 μM three primers (SH1-F1, SH1-F2 and SH1-R); 0.2 μl of Taq DNA polymerization Enzyme, 2.0 μl of template DNA; 13.8 μl of ddH2O.

[0041] The PCR reaction program was: pre-denaturation at 94°C for 5 min; denaturation at 94°C for 45 sec, annealing at 48°C for 30 sec, extension at 72°C for 1 min, and 34 cycles; finally, the amplified product was obtained after extension at 72°C for 5 min.

[0042] (3) Detection of amplification products

[0043] The amplified produc...

example 2

[0047] Example 2 Using functional marker SH1 to detect "three-line" rice breeding parents

[0048] (1) Genomic DNA of 332 restorer lines, 84 sterile lines and 81 maintainer lines were extracted respectively: rice leaves were taken respectively, and rice genomic DNA was obtained by TPS simple method.

[0049] (2) PCR amplification

[0050]The PCR reaction system is a 20 μl reaction system: 2.0 μl of 10×PCR buffer; 0.5 μl of 10 mM dNTPs; 0.5 μl of 10 μM three primers (SH1-F1, SH1-F2 and SH1-R); 0.2 μl of Taq DNA polymerization Enzyme, 2.0 μl of template DNA; 13.8 μl of ddH 2 O.

[0051] The PCR reaction program was: pre-denaturation at 94°C for 5 min; denaturation at 94°C for 45 sec, annealing at 48°C for 30 sec, and extension at 72°C for 1 min, 34 cycles; finally, the amplified product was obtained after extension at 72°C for 5 min.

[0052] (3) Detection of amplification products

[0053] The amplified product was electrophoresed in a 6% (w / w) polyacrylamide denaturing gel...

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Abstract

The invention discloses a functional marker of a rice seed shattering gene qSH1, and application of the functional marker. Three primers, namely SH1-F1, SH1-F2 and SH1-R, are designed and synthesizedaccording to mononucleotide mutation causing seed shattering gene qSH1 phenotypic difference; the three primers perform amplification on rice DNA in the same PCR system; and the amplification productis subjected to electrophoresis detection to detect the genotype of the seed shattering gene. The functional marker can screen and identify the seed shattering property of a large amount of rice germplasm resources rapidly and accurately, can improve the seed selection efficiency and can be used for performing early auxiliary selection on the seed shattering property of the rice so as to accelerate the seed selection progress.

Description

technical field [0001] The invention belongs to the field of agricultural biology, and in particular relates to a functional marker of rice shattering gene qSH1 and its application. Background technique [0002] The threshing resistance trait is one of the main breeding goals for breeding new rice varieties suitable for mechanized planting. Breeding rice materials with suitable threshing resistance is of great significance for reducing the yield reduction caused by shattering during rice harvest. And clarifying the degree of difficulty of threshing of breeding parent materials can effectively improve the efficiency of breeding. [0003] According to the existing research, the rice shattering main QTL qSH1 resolved 68.6% of the phenotypic variation. qSH1 encodes a BEL1-type homeotic protein, and a SNP in the upstream 12kb of the open reading frame of the gene causes the difference in shattering. The SNP is a G base in the easy-shattering varieties Kasalath and Oryza rufipog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/6895
Inventor 张泽民梁嘉燕邹虎成谢庆军陈雄辉
Owner SOUTH CHINA AGRI UNIV
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