A new pigeon adenovirus real-time fluorescent quantitative PCR detection kit
A technology of real-time fluorescence quantification and detection kit, which is applied in the field of animal epidemiology to achieve the effect of rapid detection, good repeatability and strong specificity
Inactive Publication Date: 2020-08-14
INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
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Problems solved by technology
At present, there are no real-time fluorescence quantitative PCR detection primers, probes and methods related to pigeon new adenovirus research reports at home and abroad. The establishment of the present invention can fill the gaps in related fields at home and abroad
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Embodiment 1
[0026] 1. Related test pathogens
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The invention relates to a novel real-time fluorescence quantification PCR detection kit for pigeon adenovirus and belongs to the field of epizootiology. A detection method comprises design of specific primers and probe sequence, establishment of standard plasmid, establishment and optimization of a real-time fluorescence quantification PCR amplification method, extraction of a DNA sample and result detection and judgment. The method for establishing the primer and probe for real-time fluorescence quantification PCR detection on novel pigeon adenovirus according to the invention has the advantages of high sensitivity, high stability, high specificity and high repeatability for detecting novel pigeon adenovirus, is capable of detecting at least 31.2 copies and can be applied to infection detection on novel pigeon adenovirus in a clinic sample.
Description
technical field [0001] The invention relates to a real-time fluorescent quantitative PCR detection kit for a new type of pigeon adenovirus, which belongs to the field of animal infectious diseases. Background technique [0002] According to the classification of the International Committee on Taxonomy of Viruses (ICTV), Adenoviridae (Adenoviridae) consists of 5 genera, namely AT-rich Adenovirus ( Atadenovirus ), avian adenovirus ( Aviadenovirus ), fish adenovirus ( Ichtadenovirus ), mammalian adenovirus ( Mastadenovirus ) and sialidaseviruses ( Siadenovirus ). Among them, there are 5 species of AT-rich adenovirus, which are bovine adenovirus type D ( Bovine adenovirus D), sheep adenovirus type D ( Ovine adenovirus D), kangaroo adenovirus type A ( Possum adenovirus A), snake adenovirus type A ( Snake adenovirus A) and duck adenovirus type A ( Duck adenovirus A) (also known as duck adenovirus type 1, Duck adenovirus 1). [0003] The adenovirus virus particle...
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IPC IPC(8): C12Q1/70C12Q1/6851C12R1/93
Inventor 万春和黄瑜程龙飞陈翠腾刘荣昌施少华傅光华陈红梅傅秋玲
Owner INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI