An electrochemical working electrode for sensing tyrosine

A sensing electrode, electrochemical technology, applied in scientific instruments, material analysis by electromagnetic means, instruments, etc., can solve the problems of low sensitivity, narrow linear range, and high preparation cost, and achieve high sensitivity, wide linear range, and preparation. low cost effect

Inactive Publication Date: 2019-12-24
HARBIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The invention belongs to the technical field of electrochemical sensors, and aims to solve the problems of narrow linear range, low sensitivity and high preparation cost of enzyme-free electrochemical sensors currently used to detect tyrosine, and provide a sensor for tyrosine electrochemical working electrode

Method used

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  • An electrochemical working electrode for sensing tyrosine
  • An electrochemical working electrode for sensing tyrosine
  • An electrochemical working electrode for sensing tyrosine

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Experimental program
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specific Embodiment approach 1

[0021] Specific embodiment one: This embodiment is a kind of electrochemical working electrode that is used for sensing tyrosine to be by ITO electrode and the carbon nanotube / chitosan- The composite film of copper chelate is composed of a polyethyleneimine layer, a phosphomolybdovanadium heteropolyacid-doped multi-walled carbon nanotube layer, and a chitosan-copper chelate layer from the ITO electrode layer to the outside. The carbon nanotube layer doped with phosphomolybdovanadium heteropolyacid and the chitosan-copper chelate layer are one cycle, and the cycle is n times, marked as PEI / [P 2 Mo 16 V 2 -MWCNTs / CTS-Cu] n .

specific Embodiment approach 2

[0022] Embodiment 2: A preparation method of an electrochemical working electrode for sensing tyrosine is carried out in the following steps.

[0023] 1. Preparation of carbon nanotube solution doped with phosphorus molybdenum vanadium heteropolyacid:

[0024] ① Dissolve multi-walled carbon nanotubes in deionized water under ultrasonic stirring at a frequency of 80Hz-100Hz, and continue ultrasonication for 10h-12h to obtain solution A;

[0025] ②Under the condition of magnetic stirring with a stirring speed of 40r / min~60r / min, the Dawson type polyoxometalate P 2 Mo 16 V 2 Dissolve in deionized water, and continue to stir for 5 to 10 minutes to obtain solution B;

[0026] ③ Add solution B dropwise to solution A at a rate of 5 mg / s to 10 mg / s, and ultrasonically stir for 12 hours to 24 hours at a temperature of 25 to 40 °C, with an ultrasonic frequency of 80 Hz to 100 Hz, to obtain phosphomolybdenum vanadium Polyacid-doped carbon nanotube solution, denoted as P 2 Mo 16 V ...

specific Embodiment approach 3

[0057] Embodiment 3: This embodiment differs from Embodiments 1 to 2 in that the ratio of the mass of multi-walled carbon nanotubes to the volume of deionized water in solution A in step ① is 30 mg:10 mL. Other steps and parameters are the same as those in the specific embodiment 1 to 2.

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Abstract

The invention disclose a tyrosine-sensing electrochemical working electrode, in particular relates to a tyrosine-sensing electrochemical working electrode based on a molybdovanadophosphoric heterpolyacid-doped carbon nanotube / chitosan-copper chelate, belongs to the technical field of electrochemical sensors, and aims at solving the problems of narrow linear ranges, low sensitivity and high preparation cost of tyrosine detection non-enzyme electrochemical sensors in the prior art. The tyrosine-sensing electrochemical working electrode comprises an ITO electrode and a PEI / [P2Mo16V2-MWCNTs / CTS-Cu]n film externally coating the ITO electrode, a molybdovanadophosphoric heterpoly acid-doped carbon nanotube / chitosan-copper chelate layer is a cycle, the number of the cycles is n, and n = 1-10. The electrochemical sensor constructed based on the working electrode has good tyrosine sensing property, the linear range is 4.99 * 10<-8>M-6.88 * 10<-5>M (M:mol / L), and the sensitivity is 0.206 mu M / mu A.

Description

technical field [0001] The invention belongs to the technical field of electrochemical sensors, in particular to an electrochemical working electrode for sensing tyrosine. Background technique [0002] Tyrosine is one of the 20 kinds of amino acids that make up protein molecules. It is the precursor for the synthesis of dopamine, thyroxine and neurotransmitters in the mammalian central nervous system. It is essential for establishing and maintaining the nutritional balance of the human body. Low levels of tyrosine in the human body can lead to albinism and alkaline phosphaturia, while high levels of tyrosine can lead to abnormal sister chromatid exchange in humans. Therefore, it is of great practical significance to develop a fast and simple method for sensing tyrosine. [0003] At present, the common methods for detecting tyrosine include fluorescence spectrometry, high performance liquid chromatography and high performance gas chromatography, etc., but these methods have ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327
Inventor 马慧媛焦佳谭立超庞海军
Owner HARBIN UNIV OF SCI & TECH
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