Method for distinguishing ureaplasma urealyticum and ureaplasma parvum in ureaplasma by using culture method

Abstract

The invention discloses a method for distinguishing ureaplasma urealyticum and ureaplasma parvum in ureaplasma by using a culture method. The method comprises the following steps: firstly, confirmingtwo detection micro-pores, namely a pore A and a pore B; putting a proper amount of glucose or / and a urease inhibitor into the pore A, putting a manganese-magnesium solution into the pore B, drying, adhering the glucose or / and the urease inhibitor to the inner wall of the pore A, and adhering manganese ions and magnesium ions to the inner wall of the pore A; putting a sample to be tested into a ureaplasma culture medium, uniformly mixing, respectively putting into the pore A and the pore B, and culturing for 24-48 hours at 37 DEG C; and according to the color change situations of the pore A and the pore B, confirming whether the sample has ureaplasma urealyticum and ureaplasma parvum or not. A kit which is relatively convenient to use is prepared according to the method. The method disclosed by the invention is simple to operate, whether a specimen contains the ureaplasma urealyticum and the ureaplasma parvum or not can be precisely distinguished at one step, meanwhile, the method canbe implemented together with drug allergy detection, and identification and drug allergy results can be achieved; and identification results are visible and accurate, and clinical popularization and application are facilitated.

Description

technical field [0001] The invention relates to biological detection technology, in particular to a method for distinguishing Ureaplasma urealyticum and Ureaplasma micronum in Ureaplasma by a culture method. Background technique [0002] Ureaplasma (Ureaplasma) was first isolated from human reproductive tract specimens as a T strain in 1954, and in 1974, it was named as a new species - Ureaplasma urealyticum by Shepard MC et al. Ureaplasma urealyticum is divided into two biological groups: Parvo group and T960 group, which can be further divided into 14 serotypes, Parvo group consists of serotypes 1, 3, 6, and 14, and T960 group includes 2, 4, There are ten serotypes including 5, 7, 8, 9, 10, 11, 12, and 13. The appearance of the colonies formed by the culture of these two groups is consistent, and the division of biogroups can only be based on the differences between the genomes, which requires the use of nucleic acid detection methods. It is also stated in the literature...

AI Technical Summary

Problems solved by technology

Single-primer PCR cannot distinguish between UU and UP, and what is detected is UU and UP; multi-primer multiplex PCR and gene sequencing can distinguish UU and UP, but the operation is too complicated and not suitable for clinical use, and drug sensitivity testing cannot be performed at the same time

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