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Purification technology for kudzuvine root extract

A kudzu root extract and process technology, which is applied in the field of purification technology of kudzu root extract, can solve the problems of complex kudzu root purification process, not environmentally friendly, time-consuming, etc., and achieve the effects of easy popularization and application, time saving and simple method

Inactive Publication Date: 2018-01-26
ZHEJIANG JIANFENG PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention proposes a purification process of kudzu root extract, which solves the problems of complex, time-consuming, and environmental-friendly purification process of kudzu root in the prior art

Method used

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Examples

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Effect test

preparation example Construction

[0020] The preparation method of the pueraria root crude extract powder in the following examples is as follows: take the pueraria root, heat extract 3-5 times with 80% ethanol, concentrate, and spray dry to obtain the pueraria root crude extract powder. This method is not intended to limit the scope of the invention.

[0021] All reagents used in the following examples are commercially available.

Embodiment 1

[0023] A purification process of Pueraria root extract, comprising the steps of:

[0024] Get 1 kilogram of kudzu root crude extract powder (puerarin content is 18.1%), add 20 kilograms of waters and dissolve, be that the membrane ultrafiltration equipment of 3500 carries out ultrafiltration with membrane molecular weight, keep solution temperature 50 ℃ during ultrafiltration, collect filtrate, Concentrate and spray-dry to obtain 330 grams of powder (the puerarin content detected by HPLC is 37.3%). The retentate is concentrated and spray-dried to obtain 523 grams of powder (the total flavonoids of Pueraria root detected by UV is 21.2%).

Embodiment 2

[0026] A purification process of Pueraria root extract, comprising the steps of:

[0027] Get 1.5 kilograms of kudzu root crude extract powder (puerarin content is 17.3%), add 30 kilograms of 20% concentration ethanol solutions and dissolve, carry out ultrafiltration with the membrane ultrafiltration equipment that membrane molecular weight is 5000, keep solution temperature 40 ℃ during ultrafiltration, The filtrate was collected, concentrated, spray-dried to obtain 520 grams of powder (HPLC detection of puerarin content was 36.2%), the retentate was concentrated, and spray-dried to obtain 763 grams of powder (UV detection of Pueraria total flavonoids was 20.1%).

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Abstract

The invention provides a purification technology for kudzuvine root extract. The purification technology provided by the invention comprises the step of carrying out ultrafiltration on kudzuvine rootcrude extract by virtue of an ultrafiltration membrane, wherein rated molecular weight of the ultrafiltration membrane is 20000-1000. The purification technology provided by the invention has the advantages that an ultrafiltration technology is adopted for obtaining puerarin and pueraria flavones, the method is simple, time is shortened, and environmental friendliness is achieved; the method reduces loss of effective ingredients of kudzuvine root; and the method also innovatively adopts the ultrafiltration membrane with the rated molecular weight of 20000-1000, the ultrafiltration technology is applied to a kudzuvine root purification technology, and industrialization popularization and application are convenient.

Description

technical field [0001] The invention relates to the technical field of plant extraction and purification, in particular to a purification process of kudzu root extract. Background technique [0002] Pueraria is a leguminous plant, distributed in most parts of my country, and likes a warm and humid environment. Its varieties are mainly divided into two types: Pueraria lobata and Pueraria lobata. Pueraria lobata is rich in starch, which is an important food material and food additive; Pueraria mirifica is rich in isoflavones, which has important pharmacological functions and medicinal value. Ancient Chinese medical monographs "Shen Nong's Materia Medica", "Treatise on Febrile and Miscellaneous Diseases", "Golden Plaque Summary", "Compendium of Materia Medica" and Japan's "Niyangshijian" and "Waka Food Materia Medica" and other famous works have records of kudzu root treatment of diseases . Pueraria lobata contains a variety of active ingredients, among which pueraria puerar...

Claims

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Application Information

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IPC IPC(8): A61K36/488
Inventor 黄金龙林军庞雄施存元伊胜荣何新华
Owner ZHEJIANG JIANFENG PHARM CO LTD