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Outdoor ambrostoma quadriimpressum motschulsky trap design for green plant resources based on AquaOBP4

A technology of green plants and traps, which is applied in the device, application, animal husbandry and other directions of catching or killing insects, can solve the problems of low efficiency, laborious and high cost, and achieves less labor and labor input, good control effect and environmental protection. low impact effect

Inactive Publication Date: 2018-02-02
NORTHEAST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are disadvantages such as low efficiency, laborious and laborious, narrow scope of application, environmental pollution, and high cost.
None of the four methods mentioned above can safely and effectively control the damage of elm beetle to elm trees, so it is particularly important to explore new control methods

Method used

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  • Outdoor ambrostoma quadriimpressum motschulsky trap design for green plant resources based on AquaOBP4
  • Outdoor ambrostoma quadriimpressum motschulsky trap design for green plant resources based on AquaOBP4
  • Outdoor ambrostoma quadriimpressum motschulsky trap design for green plant resources based on AquaOBP4

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Experimental program
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Effect test

Embodiment Construction

[0048] The examples in the scheme are used to illustrate the present invention, but are not used to limit the scope of the present invention.

[0049] 1. Determination of the composition of the lure compound

[0050] 1. Extraction of total RNA from the antennae of Ulmus chinensis, synthesis of cDNA, design of primers, PCR amplification, construction of pET28a-OBP4 expression vector, induction of expression, protein purification and quantitative analysis, and competitive binding of ligands and fluorescent probes .

[0051] 2. Using the sequence of AquaOBP4 for homologous template screening. The model construction of AquaOBP4 was carried out on the On-line Swiss-model software; the molecular structures of the five compounds with binding ability were constructed in Sketch mode, and molecular docking was carried out with them. In order to explore the reliability of the constructed protein spatial conformation model, two analogues were selected for molecular docking.

[0052] 3....

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Abstract

The invention belongs to the technical field of agricultural disease and pest defence, and particularly relates to an outdoor ambrostoma quadriimpressum motschulsky trap device for green plant resources. The device is mainly composed of a rain cap, transparent baffles, a pest collecting device and a luring agent storage pot, and the device is characterized by determining the component of a luringagent in the luring agent storage pot, wherein an fluorescence competitive binding experiment is conducted on 40 relative kinds of compounds in a habitat of ambrostoma quadriimpressum motschulsky by people, 5 kinds of the compounds are screened out preliminarily, then by building an AquaOBP4 molecular structural model, three kinds of the compounds are further determined according to the space structure and binding characteristics of AquaOBP4, and then through EAG and ethology verification, the compound which is strongly bonded with the AquaOBP4 is finally determined as the Dibutyl phthalate; the chemical agent is stored in the luring agent storage pot, the ambrostoma quadriimpressum motschulsky swoop over when stimulated by the smell of the luring agent, hit the transparent baffles successfully and then fall into the pest collecting device below. According to the device, the operation cost is low, no pollution is caused to the environment, influence from the environment is little, a good control effect can be achieved by large-scale prevention and treatment, and not only can the device be used individually, but also a comprehensive prevention and treatment system can be formed.

Description

[0001] Technical field: the invention belongs to the technology of preventing agricultural diseases and insect pests, and in particular relates to a field trapping device for the elm purple leaf beetle. Background technique [0002] Elm is a deciduous tree belonging to the genus Ulmus in the family Ulmaceae. Because of its rapid growth, strong fecundity, and wide adaptability, it is resistant to cold, drought, barrenness, and salt and alkali. It is the main tree species for afforestation, windbreak and sand fixation, and ecological protection in the north. In recent years, due to the rampant occurrence of Ulmus elm, the normal growth of elm trees in Northeast my country has been seriously affected, and huge losses have been caused to the economy and ecology. [0003] The elm purple leaf beetle belongs to the family Coleoptera and is a monophagous pest that harms elm trees. It is distributed in Heilongjiang, Jilin, Liaoning, Hebei, Guizhou and other provinces and Inner Mongolia...

Claims

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Application Information

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IPC IPC(8): A01M1/02
CPCA01M1/026
Inventor 王寅亮刘雨林任炳忠
Owner NORTHEAST NORMAL UNIVERSITY
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