Outdoor ambrostoma quadriimpressum motschulsky trap design for green plant resources based on AquaOBP4
A technology of green plants and traps, which is applied in the device, application, animal husbandry and other directions of catching or killing insects, can solve the problems of low efficiency, laborious and high cost, and achieves less labor and labor input, good control effect and environmental protection. low impact effect
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[0048] The examples in the scheme are used to illustrate the present invention, but are not used to limit the scope of the present invention.
[0049] 1. Determination of the composition of the lure compound
[0050] 1. Extraction of total RNA from the antennae of Ulmus chinensis, synthesis of cDNA, design of primers, PCR amplification, construction of pET28a-OBP4 expression vector, induction of expression, protein purification and quantitative analysis, and competitive binding of ligands and fluorescent probes .
[0051] 2. Using the sequence of AquaOBP4 for homologous template screening. The model construction of AquaOBP4 was carried out on the On-line Swiss-model software; the molecular structures of the five compounds with binding ability were constructed in Sketch mode, and molecular docking was carried out with them. In order to explore the reliability of the constructed protein spatial conformation model, two analogues were selected for molecular docking.
[0052] 3....
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