Maize PILNCR1 and application thereof in regulating and detecting maize tolerance to low-phosphorus stress

A low-phosphorus-resistant, sequence-listed technology that can be used in applications, DNA/RNA fragments, recombinant DNA technology, etc.

Active Publication Date: 2018-02-02
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the development of high-throughput sequencing technology, a large number of lncRNAs have been found in Arabidopsis, rice, corn, cotton and other plants, but the functions of these lncRNAs are still unclear
Maize is the most widely planted food

Method used

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  • Maize PILNCR1 and application thereof in regulating and detecting maize tolerance to low-phosphorus stress
  • Maize PILNCR1 and application thereof in regulating and detecting maize tolerance to low-phosphorus stress
  • Maize PILNCR1 and application thereof in regulating and detecting maize tolerance to low-phosphorus stress

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1. Maize PILNCR1 inhibits the shearing of PHO2 by miR399

[0055] This example provides a LncRNA derived from the low phosphorus sensitive inbred line 31778, its name is PILNCR1, the sequence of PILNCR1 is the 1-649th position of sequence 1 in the sequence table, and the first position of sequence 1 is the 5' of PILNCR1 end. There is a sequence complementary to the mature sequence of miR399 at the 404-428bp of the 5' end of PILNCR1, and there is a 3bp protrusion between the 10th and 11th bases of miR399 in PILNCR1, as shown in figure 1 shown.

[0056] In order to study the effect of PILNCR1 on miR399 and miR399 target gene PHO2 in maize, the inventors combined PILNCR1 full length, PILNCR1 del (remove the sequence complementary to miR399), the full length of miR399 and PHO2 were respectively connected to the pCPB vector, and transformed into protoplasts of tobacco and maize, and the expression of PHO2 was detected. The specific operation method is as follows: ...

Embodiment 2

[0090] Example 2, the expression of PILNCR1 in maize inbred lines with different low phosphorus tolerance characteristics

[0091] Determination of low-phosphorus-sensitive maize inbred lines (31778, FR19, 1538, JI419) and low-phosphorus-tolerant maize inbred lines (CCM454, XI14, HAI9-21, Q1261) under normal and low-phosphorus stress conditions by absolute quantitative method The expression of PILNCR1 in .

[0092] Normal treatment (normal phosphorus condition) and low phosphorus treatment (low phosphorus stress condition) methods are as follows:

[0093] Select plump corn seeds of the same size, soak them with 3% NaClO for 20 minutes, and rinse them with sterilized distilled water for 3-4 times. Soak the seeds in distilled water at room temperature for 6 hours, and then bury the seeds in quartz sand (soak the quartz sand with hydrochloric acid overnight, rinse with distilled water 2-3 times); culture at 28°C for 6-7 days, when one leaf and one heart grow (about day), select...

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Abstract

The invention discloses maize PILNCR1 and application thereof in regulating and detecting maize tolerance to low-phosphorus stress. The maize PILNCR1 disclosed in the invention is any one of the following a1) to a4): a1): an RNA represented by position 1 to 649 of NO:1 in a sequence table; a2): an RNA represented by NO:1 in the sequence table; a3): an RNA having 75% or above identity to the RNA defined in a1) or a2); a4): an RNA hybridizing with the RNA defined in a1) or a2) under stringent conditions. Experiments show that PILNCR1 can bind to miR399 to inhibit cleavage of target gene PHO2 bymiR399 and further regulate ability of plants to tolerate low-phosphorus stress by reducing expression of PHO2. PILNCR1 plays an important role in plant adaptation to low-phosphorus stress and can beused to regulate and test the ability of the plants to adapt to the low-phosphorus stress.

Description

technical field [0001] The invention relates to the field of biotechnology, corn PILNCR1 and its application in regulating and detecting corn tolerance to low phosphorus stress. Background technique [0002] Phosphorus is one of the mineral elements necessary for plant growth and development, and participates in various forms of important physiological and biochemical processes in plants such as photosynthesis, respiration, and enzymatic reactions. Although the total phosphorus content in the soil is high, phosphorus is often fixed in the soil by forming insoluble compounds with cations in the soil, which leads to very low bioavailability of phosphorus in the soil. Plants will reduce the damage caused by low phosphorus stress through complex morphological changes and physiological and biochemical reactions. When plants are subjected to low phosphorus stress, the plants will become short, and the plant height can be used as an indicator to evaluate the ability of plants to t...

Claims

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Application Information

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IPC IPC(8): C12N15/113C12N15/82A01H5/00C12Q1/6895A01H6/46
CPCC12N15/113C12N15/8273C12N2310/141C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 李文学杜庆国王开
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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