Maize pilncr1 and its application in regulation and detection of maize tolerance to low phosphorus stress

Abstract

The invention discloses maize PILNCR1 and application thereof in regulating and detecting maize tolerance to low-phosphorus stress. The maize PILNCR1 disclosed in the invention is any one of the following a1) to a4): a1): an RNA represented by position 1 to 649 of NO:1 in a sequence table; a2): an RNA represented by NO:1 in the sequence table; a3): an RNA having 75% or above identity to the RNA defined in a1) or a2); a4): an RNA hybridizing with the RNA defined in a1) or a2) under stringent conditions. Experiments show that PILNCR1 can bind to miR399 to inhibit cleavage of target gene PHO2 bymiR399 and further regulate ability of plants to tolerate low-phosphorus stress by reducing expression of PHO2. PILNCR1 plays an important role in plant adaptation to low-phosphorus stress and can beused to regulate and test the ability of the plants to adapt to the low-phosphorus stress.

Description

technical field [0001] The invention relates to the field of biotechnology, corn PILNCR1 and its application in regulating and detecting corn tolerance to low phosphorus stress. Background technique [0002] Phosphorus is one of the mineral elements necessary for plant growth and development, and participates in various forms of important physiological and biochemical processes in plants such as photosynthesis, respiration, and enzymatic reactions. Although the total phosphorus content in the soil is high, phosphorus is often fixed in the soil by forming insoluble compounds with cations in the soil, which leads to very low bioavailability of phosphorus in the soil. Plants will reduce the damage caused by low phosphorus stress through complex morphological changes and physiological and biochemical reactions. When plants are subjected to low phosphorus stress, the plants will become short, and the plant height can be used as an indicator to evaluate the ability of plants to t...

AI Technical Summary

Problems solved by technology

With the development of high-throughput sequencing technology, a large number of lncRNAs have been found in Arabidopsis, rice, corn, cotton and other plants, but the functions of these lncRNAs are still unclear

Maize is the most widely planted food and feed crop in the world. The research on lncRNA in maize is currently focused on the discovery of new lncRNAs through transcriptome analysis. The regulatory mechanism and related biological functions of these lncRNAs need to be further studied

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