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Biological treatment method of copper polluted seawater

A biological treatment and copper pollution technology, applied in the field of biological treatment of copper-contaminated seawater, can solve problems such as copper pollution, achieve the effect of improving adsorption capacity, enhancing quantity and activity, and preventing interference

Active Publication Date: 2018-02-09
MARINE FISHERIES RES INST OF ZHEJIANG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, there is a need for a living organism with the characteristics of large adsorption capacity, strong selectivity, high efficiency, and low cost to adsorb copper to solve the problem of copper pollution.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A biological treatment method for copper polluted seawater, comprising the following steps:

[0031] (1) Cultivation of microorganisms: Inoculate the microorganisms into the culture medium for cultivation;

[0032](2) Remediation of heavy metal pollution: Add the microorganisms obtained in step (1) into heavy metal-contaminated seawater to repair heavy metal pollution;

[0033] Wherein, the microorganisms are Chlorella chinensis and Candida catenida, wherein the quantity ratio of Chlorella chinensis and Candida catenida is 4:5. The described Chlorella chinensis is cultivated by the following method: inoculate the chlorella chinensis on the liquid medium, culture at a temperature of 30° C., and cultivate under light conditions with a light intensity of 8000 lx, and irradiate 12 hours of light every day, at a speed of Cultivate on a shaker at 120rpm for 18h, collect and resuspend by centrifugation, and adjust the OD600 of the algae liquid of Chlorella sinensis to 0.8; th...

Embodiment 2

[0040] A biological treatment method for copper polluted seawater, comprising the following steps:

[0041] (1) Cultivation of microorganisms: Inoculate the microorganisms into the culture medium for cultivation;

[0042] (2) Remediation of heavy metal pollution: Add the microorganisms obtained in step (1) into heavy metal-contaminated seawater to repair heavy metal pollution;

[0043] Wherein, the microorganisms are Chlorella chinensis and Candida catenida, wherein the quantity ratio of Chlorella chinensis and Candida catenida is 4:5. The described Chlorella chinensis is cultivated by the following method: inoculate the chlorella chinensis on the liquid medium, culture at 25° C., cultivate under light conditions, light intensity is 6000 lx, and irradiate 11 hours of light every day. The rotating speed is 100rpm shaker and cultivated for 18h, centrifuged to collect and resuspend, and adjust the OD600 of the algae liquid of Chlorella sinensis to 0.8; the Candida catenidae is c...

Embodiment 3

[0050] A biological treatment method for copper polluted seawater, comprising the following steps:

[0051] (1) Cultivation of microorganisms: Inoculate the microorganisms into the culture medium for cultivation;

[0052] (2) Remediation of heavy metal pollution: Add the microorganisms obtained in step (1) into heavy metal-contaminated seawater to repair heavy metal pollution;

[0053] Wherein, the microorganisms are Chlorella chinensis and Candida catenida, wherein the quantity ratio of Chlorella chinensis and Candida catenida is 4:5. The described Chlorella chinensis is cultivated by the following method: inoculate the chlorella chinensis on the liquid culture medium, culture at 28° C., cultivate under light conditions, light intensity is 7000 lx, irradiate 11 hours of light every day, in The rotational speed is 120rpm shaker culture 24h, centrifugation collects and resuspends, and adjusts the OD600 of the algae liquid of Chlorella sinensis to be 1.0; The described Candida ...

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PUM

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Abstract

The invention relates to the technical field of environmental protection, in particular to a biological treatment method of copper polluted seawater. The biological treatment method of the copper polluted seawater is characterized by comprising the following steps: inoculating microorganisms into a culture medium, performing cultivation, adding the microorganisms into the heavy metal polluted seawater and performing heavy metal polluted repair, wherein the microorganisms are one or a combination of more of bacillus, aspergillus flavus, Chinese chlorella, prorocentrum donghaiense and candida catenulata. The biological treatment method of the copper polluted seawater has the characteristics of large adsorption capacity, high selectivity, high efficiency and low cost; furthermore, a method for embedding the microorganisms is adopted, the treatment capability on the copper heavy metal in the seawater is improved, and the stability is improved.

Description

technical field [0001] The invention relates to the technical field of environmental protection, in particular to a biological treatment method for copper-contaminated seawater. Background technique [0002] Due to the wanton discharge of various waste water, serious pollution of soil, rivers and other environments has been caused, and heavy metal pollution is one of them. Heavy metal pollution in rivers inevitably destroys the biological environment of estuaries and coastal tidal flat waters, so that the enrichment of tidal flat aquatic animals causes heavy metals in the food chain to exceed the standard, thereby affecting human health. [0003] Copper compounds exist in a monovalent or divalent state. In natural water, the amount of dissolved copper decreased with the increase of pH value. When the pH value is 6-8, the solubility is 50-500 μg / L. When the pH value is less than 7, basic copper carbonate [Cu 2 (OH) 2 CO 3 ] has the maximum solubility; when the pH value ...

Claims

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Application Information

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IPC IPC(8): C02F3/34C02F3/32C12N1/20C12N1/14C12N1/12C12R1/89C12R1/72C12R1/67C02F101/20C02F103/08
CPCC02F3/322C02F3/325C02F3/347C02F3/348C02F2101/20C02F2103/08C12N1/12C12N1/14C12N1/20
Inventor 郭远明李铁军刘琴王范盛尤炬矩宋凯
Owner MARINE FISHERIES RES INST OF ZHEJIANG
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