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Protecting agent and preservation method for total microorganisms of sample and total DNA

A technology of microorganisms and protective agents, applied in the field of microorganism preservation, can solve the problem of inability to quickly and effectively preserve the total microorganisms and their total DNA of the sample, and achieve the effects of facilitating storage and transportation, inhibiting DNase activity, and maintaining integrity.

Active Publication Date: 2018-02-16
INST OF MICROBIOLOGY JIANGXI ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore, the technical problem to be solved by the present invention is to overcome the inability to quickly and effectively preserve the sample total microorganisms and their total DNA in environmental samples in the prior art, thereby providing a sample total microorganisms and their total DNA with a long storage period and good preservation effect. Protective agent and preservation method of DNA

Method used

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  • Protecting agent and preservation method for total microorganisms of sample and total DNA
  • Protecting agent and preservation method for total microorganisms of sample and total DNA
  • Protecting agent and preservation method for total microorganisms of sample and total DNA

Examples

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Embodiment 1

[0029] This embodiment provides a protective agent for total microorganisms and total DNA of samples, including the following raw materials: 15g of glycerol, 12g of sucrose, 9g of sodium glutamate, 2g of polyethylene glycol, 0.5g of EDTA disodium salt, and 7g of sodium citrate , water 60g and liquid paraffin 150g.

[0030] The raw materials are weighed according to the above formula of the protective agent for the total microorganisms and the total DNA of the sample, and then the weighed raw materials are mixed together and thoroughly mixed to obtain the product.

Embodiment 2

[0032] The present embodiment provides a protective agent for total microorganisms and total DNA thereof, including the following raw materials: 25 g of glycerol, 8 g of sucrose, 5 g of sodium glutamate, 0.5 g of polyethylene glycol, 0.1 g of EDTA disodium salt, citric acid Sodium 3g, water 56g and liquid paraffin 50g.

[0033] The raw materials are weighed according to the above formula of the protective agent for the total microorganisms and the total DNA of the sample, and then the weighed raw materials are mixed together and thoroughly mixed to obtain the product.

Embodiment 3

[0035] The present embodiment provides a protective agent for total microorganisms and total DNA thereof, including the following raw materials: 20 g of glycerol, 10 g of sucrose, 7 g of sodium glutamate, 1 g of polyethylene glycol, 0.37 g of disodium EDTA, and sodium citrate 5g, water 56.63g and liquid paraffin 100g.

[0036] The raw materials are weighed according to the above formula of the protective agent for the total microorganisms and the total DNA of the sample, and then the weighed raw materials are mixed together and thoroughly mixed to obtain the product.

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Abstract

The invention discloses a protecting agent and a preservation method for total microorganisms of a sample and total DNA. The protecting agent for total microorganisms of the sample and total DNA comprises glycerinum, cane sugar, sodium glutamate, polyethylene glycol, EDTA disodium, sodium citrate, water and liquid paraffin. The preservation method for total microorganisms of the sample and total DNA comprises the following steps: adding a sample liquid onto the surface of a filter membrane for suction filtration; taking down the filter membrane; by taking the membrane surface containing the sample as an inner membrane surface, folding the filter membrane; placing the same in a sterile bag; injecting the protecting agent of the total microorganisms of the sample and the total DNA thereof till the whole filter membrane is infiltrated; and sealing the filter membrane after exhausting air and freezing and preserving the same.

Description

technical field [0001] The invention relates to the field of microorganism preservation, in particular to a protective agent and a preservation method for total microorganisms and total DNA of a sample. Background technique [0002] In the past, in the investigation of the microbial community of the ecological environment, limited to the analysis techniques and conditions at that time, the collected samples were often not paid attention to and retained after the end of the project. The most complete record of the microbial flora information at the sampling point is specific, unique and irreproducible. Due to technical limitations, it is not possible to perform real-time rapid micro-processing on the samples on site, and the collected samples often occupy a large volume, which is not easy to store and transport, and as the transport time increases during the transport process, the microorganisms in the samples will increase. Changes or even cross-contamination occur, resulti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/04C12N15/10
CPCC12N1/04C12N15/10
Inventor 涂祖新郑国华贺伟华张莉莉刘卓荣
Owner INST OF MICROBIOLOGY JIANGXI ACADEMY OF SCI