A kind of efficient broad bean hybridization method

Abstract

The invention discloses an efficient broad bean hybridization method, and belongs to the technical field of plant hybridization. The method comprises the following steps of (1) castration: selecting hybridization flowers; peeling petals for castration; (2) pollination: selecting male parent flowers about to loose powder or just loosing powder; binding the male parent flowers onto the chapiters offemale parent flowers by transparent adhesive tapes; (3) plate sleeving; (4) branch sorting. After the stamens of the female parent flowers are removed, the male parent flowers are bound onto the female parent flowers by the transparent adhesive tapes for natural pollination; the wind blowing and rain drenching of the chapiters due to exposure can be avoided; the heat insulation effect is achieved; the male parent flowers and the female parent flowers are bound by the adhesive tapes, so that the insect inducing is avoided; the use of isolation measures is not needed; after the pollination, thedefects of secondary damage to the female parent flowers and the like by the use of isolation measures can be overcome, so that the hybridization success rate is much higher than that of a conventional pollen smearing method; the method is particularly suitable for the broad bean hybridization in southern spring cold and rainy regions.

Description

technical field [0001] The invention belongs to the technical field of plant hybridization, and in particular relates to a high-efficiency hybridization method of broad beans. Background technique [0002] Because the seed-setting rate of broad bean is generally low, in order to obtain a certain number of hybrid seeds in cross-breeding of broad bean, it is necessary to do more number of hybrid flowers. In the process of broad bean hybridization, ensuring pollination and improving hybridization efficiency is one of the important steps. In the past, until now, some scientific research institutions at home and abroad still adopt the traditional method of smearing pollen on the pistil stigma after selecting suitable hybrid flowers, using tweezers to remove the flag petals, wing petals and keel petals, and then taking out ten anthers one by one. Broad bean emasculation cross-breeding method, such as the Chinese patent "A method of broad bean cross-breeding", patent number CN 102...

AI Technical Summary

Problems solved by technology

In the past, until now, some scientific research institutions at home and abroad still adopt the traditional method of smearing pollen on the pistil stigma after selecting suitable hybrid flowers, using tweezers to remove the flag petals, wing petals and keel petals, and then taking out ten anthers one by one. Broad bean emasculation cross-breeding method, such as the Chinese patent "A method of broad bean cross-breeding", patent number CN 102150611 B, this method is easy to damage the stigma of the female parent during pollination, and because the stigma is exposed, it is vulnerable to insects and wind. Rain, especially low temperature and rainy weather after hybridization, resulting in failure of hybridization

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