Cucumber chromosome single segment substitution line culture method

Abstract

The invention discloses a method for culturing a chromosome single segment substitution line of cucumber with good powdery mildew resistance, short fruit pedicels and thick pulp. According to the method, a dwarf powdery mildew infected selfing line cucumber D8 is adopted as a female parent, a long tendril powdery mildew resistant selfing line JIN5-508 is adopted as a male parent, techniques of hybridization, backcross and marker-assisted selection are adopted to implement 12 generations of backcross breeding and 4 generations of selfing breeding continuously, then a dwarf powdery mildew resistant cucumber chromosome single segment substitution line D85109267 is successfully cultured, the substitution line has a genetic background which is nearly completely identical to that of the infectedfemale parent D8, and the sole difference is that a chromosome single segment of which the length is only 0.7cM in the disease resistant male parent JIN5-508 in the genetic background is substituted.The novel dwarf powdery mildew resistant cucumber variety D85109267 has the advantages of being short in internode, centralized in florescence, short in fruit pedicel, thick in pulp and the like andcan be adopted to prepare coeno-species for early maturing, cucumbers can be harvested relatively centrally, the early-stage yield is high, and the yield is increased by 20% or greater.

Description

technical field [0001] The invention relates to a method for cultivating a cucumber chromosome single-segment substitution line. Background technique [0002] In agricultural production, compared with conventional varieties, hybrids have the characteristics of stronger resistance and higher yield, so they often have higher cultivation benefits. About 85% of cucumber varieties are hybrids. At present, the varieties used in the production of cucumbers in my country are mainly long-fruited dense thorn cucumbers. These varieties are mainly characterized by strong growth potential and strong disease resistance. The fruit length is generally about 35 cm, and the taste is better. The largest proportion is more than 30% of the total length of the fruit), the flesh is thin, and the female rate is low. These are the main shortcomings of this cucumber variety. The medium-short fruit type cucumbers currently cultivated generally have weak growth vigor and low disease resistance, especi...

AI Technical Summary

Problems solved by technology

[0003] At present, the cucumber consumption market is showing increasingly diversified characteristics. Medium and short fruit type cucumbers are more suitable for the needs of modern family structure and individualized consumption. Breakthroughs have led to slow innovation of related varieties. Therefore, for a long time, it has not been possible to produce excellent hybrids with short fruit stems, thick flesh, early maturity, and disease resistance.