Method for identifying organic apples and common apples based on NMR metabonomics

A technology of metabolomics and apples, applied in the field of plant metabolomics, can solve problems such as damage to consumer rights and achieve effective and reliable models, high sensitivity, and accurate and reliable results

Inactive Publication Date: 2018-02-23
河南省农业科学院农业质量标准与检测技术研究所
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Problems solved by technology

In recent years, organic apples have been sought after by consumers at home and abroad, and the selling price is often several times that of ordinary apples. Therefore, in order to make profits, some unscrupulous traders often use inferior products to deceive consumers and damage their rights and interests.

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  • Method for identifying organic apples and common apples based on NMR metabonomics
  • Method for identifying organic apples and common apples based on NMR metabonomics
  • Method for identifying organic apples and common apples based on NMR metabonomics

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Embodiment Construction

[0025] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0026] A method for discriminating organic apples and common apples based on NMR metabolomics technology, comprising the steps of:

[0027] Preparation of S1 apple samples: 1 organic apple without any damage from Sanmenxia Erxianpo Green Fruit Industry Co., Ltd., 1 common apple purchased from Sanmenxia lakeside area, 5 organic apples and 5 inorganic apples randomly purchased from the market For apples, each apple was cut into 1.5 mm slices, and the cut apple s...

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Abstract

The invention provides a method for identifying organic apples and common apples based on NMR metabonomics. The method comprises the following steps: obtaining NMR fingerprints of organic apples and common apples by using a nuclear magnetic resonance technology, and analyzing the fingerprints to identify chemical compositions of the organic apples and the common apples; analyzing the chemical compositions of the organic apples and the common apples by using a supervised multidimensional statistical analysis method, namely, orthogonal partial least squares discriminant analysis (OPLS-DA); validating the reliability of the established model by two validation methods of internal cross-validation and CV-ANOVA in external validation in the OPLS-DA model, and finally solving the technical problem of identifying the organic apples and the common apples by a Score graph in the OPLS-DA model.

Description

technical field [0001] The invention relates to the field of plant metabolomics, in particular to a method for identifying organic apples and common apples based on NMR metabolomics technology. Background technique [0002] Metabonomics (metabonomics or metabolomics) is a research method that imitates the research ideas of genomics and proteomics, conducts quantitative analysis of all metabolites in organisms, and looks for the relative relationship between metabolites and physiological and pathological changes. component. Most of its research objects are small molecular substances with a relative molecular mass of less than 1000. Advanced analysis and detection technology combined with computational analysis methods such as pattern recognition and expert system are the basic methods of metabolomics research. Metabolomics is a newly developed discipline following genomics and proteomics, and is an important part of systems biology. After that, it has developed rapidly and...

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Application Information

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IPC IPC(8): G01N24/08
CPCG01N24/08
Inventor 吴绪金安莉马婧玮汪红吴家锴张军锋李通李萌马欢周娟杨国华
Owner 河南省农业科学院农业质量标准与检测技术研究所
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