Atractylenolide extract and application in CIK cell cryopreservation protection

A technology of Atractylodes lactone and extracts, which can be used in applications, preservation of human or animal bodies, alkali metal compounds, etc., can solve the problem of high cost, and achieve the effect of simple preparation method and easy industrialization

Inactive Publication Date: 2018-02-27
南京盖斯夫医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the cost of preparing pure A...

Method used

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  • Atractylenolide extract and application in CIK cell cryopreservation protection
  • Atractylenolide extract and application in CIK cell cryopreservation protection

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Experimental program
Comparison scheme
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Embodiment 1

[0025] The preparation of embodiment 1 extract

[0026] 1. Experimental materials

[0027] XDA-6 macroporous adsorption resin was purchased from Zhengzhou Qinshi Technology Co., Ltd.

[0028] 2. Experimental method

[0029] A preparation method of atractylodes atractylodes extract, comprising the steps of:

[0030] Step S1, adding acid to rot: crush Atractylodes macrocephala, spray dilute hydrochloric acid while stirring, and pile up overnight at 55°C after the powder is wet;

[0031] Step S2, ethanol extraction: extract the above-mentioned decomposed medicinal powder with an ethanol solution with a concentration of 75% by volume, collect the extract, recover the ethanol in the extract, and obtain a concentrated solution;

[0032] Step S3, macroporous resin enrichment: dilute the above concentrated solution with water, adjust the pH value to 5.5, load the sample on XDA-6 macroporous adsorption resin, and use volume percent concentration of 25%, 45%, 65%, 85% Each of the ...

Embodiment 2

[0034] The preparation of embodiment 2 extract

[0035] 1. Experimental materials

[0036] XDA-6 macroporous adsorption resin was purchased from Zhengzhou Qinshi Technology Co., Ltd.

[0037] 2. Experimental method

[0038] A preparation method of atractylodes atractylodes extract, comprising the steps of:

[0039] Step S1, adding acid to rot: smash Atractylodes macrocephala, spray dilute hydrochloric acid while stirring, and pile up overnight at 50°C after the powder is wet;

[0040] Step S2, ethanol extraction: extract the decomposed medicinal powder with an ethanol solution with a concentration of 60% by volume, collect the extract, recover the ethanol in the extract, and obtain a concentrated solution;

[0041] Step S3, macroporous resin enrichment: dilute the above concentrate with water, adjust the pH value to 5.4, load the sample on XDA-6 macroporous adsorption resin, and use volume percent concentration of 25%, 45%, 65%, 85% % ethanol solution was eluted for 6 co...

Embodiment 3

[0043] The preparation of embodiment 3 extract

[0044] 1. Experimental materials

[0045] XDA-6 macroporous adsorption resin was purchased from Zhengzhou Qinshi Technology Co., Ltd.

[0046] 2. Experimental method

[0047] A preparation method of atractylodes atractylodes extract, comprising the steps of:

[0048] Step S1, adding acid to rot: crush Atractylodes macrocephala, spray dilute hydrochloric acid while stirring, and pile up overnight at 60°C after the powder is wet;

[0049] Step S2, ethanol extraction: extract the decomposed medicinal powder with an ethanol solution with a concentration of 90% by volume, collect the extract, recover the ethanol in the extract, and obtain a concentrated solution;

[0050] Step S3, macroporous resin enrichment: dilute the above concentrated solution with water, adjust the pH value to 5.6, load the sample on XDA-6 macroporous adsorption resin, and use volume percent concentration of 25%, 45%, 65%, 85% 10 column volumes were elute...

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Abstract

The invention discloses atractylenolide extract and application in CIK cell cryopreservation protection. The atractylenolide extract is prepared by utilizing bighead atractylodes rhizome as a raw material and performing extracting and enriching, and the sum of contents of atractylenolide II and atractylenolide V in the extract is 90% or more, preferably 95% or more. The extracting and enriching step comprises S1 of acid addition for corrosion, S2 of ethanol extraction and S3 of macroporous resin enrichment. The atractylenolide extract disclosed by the invention can improve a CIK cell cryopreservation resuscitation rate, further avoids affecting killing activity of CIK cells to tumor cells, and can be prepared into a CIK cell cryopreservation solution for CIK cell cryopreservation, so as toguarantee that sufficient CIK cells can be fed back to patients after radiotherapy and chemotherapy.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to an extract of atractylodes macrolide and its application in cryopreservation protection of CIK cells. Background technique [0002] Atractylodes lactones are the main active ingredients of Atractylodes macrocephala, and atractylodes lactones with the following structure have been found so far: [0003] [0004] The applicant found that Atractylodes II and Atractylodes V have a protective effect on cryopreserved CIK cells, and has submitted related patent applications (application numbers 2017109786579 and 2017109955153). [0005] However, the cost of preparing pure Atractylodes lactone II or Atractylodes lactone V is relatively high. If it is possible to prepare an extract with Atractylodes II and Atractylodes V as the main components, the extract can be used as a cryoprotectant for CIK cells instead of Atractylodes II or Atractylodes V, reducing the Cost of production. Contents...

Claims

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Application Information

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IPC IPC(8): A01N1/02G01N30/02B01J20/26B01J20/28B01D15/08
CPCA01N1/0221B01D15/08B01J20/26B01J20/28085G01N30/02
Inventor 王莉张艳峰孙天娇
Owner 南京盖斯夫医药科技有限公司
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