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Insecticidal protein HY131c capable of resisting brown plant-hopper as well as encoding gene and application thereof

A protein and coding technology, applied in the field of insecticidal protein against rice brown planthopper and its coding gene and application, can solve the problems of promoting the population of non-target pest insects and the degree of damage

Active Publication Date: 2018-03-06
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it also inevitably promotes the population and damage degree of non-target pest insects such as brown planthopper (Chen et al.2011)

Method used

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  • Insecticidal protein HY131c capable of resisting brown plant-hopper as well as encoding gene and application thereof
  • Insecticidal protein HY131c capable of resisting brown plant-hopper as well as encoding gene and application thereof
  • Insecticidal protein HY131c capable of resisting brown plant-hopper as well as encoding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Example 1. Acquisition of Novel Insecticidal Protein Gene HY131c

[0070] 1. Cry1Ac and Cry30Fa1 protein and gene sequence analysis

[0071] The amino acid sequences of Cry1Ac and Cry30Fa1 were analyzed using the Blastp function of the NCBI website to analyze their conserved domains, and their corresponding nucleotide sequences were found. It is concluded that the Cry1Ac structural domain I is amino acid 48-251 of Cry1Ac protein, and the corresponding Cry1Ac nucleic acid coding sequence is nucleotide 142-753; domain II is amino acid 259-461 of Cry1Ac protein, and the corresponding Cry1Ac nucleic acid coding sequence is The sequence is nucleotides 775-1383; domain III is amino acids 471-609 of the Cry1Ac protein, and the nucleic acid coding sequence corresponding to Cry1Ac is nucleotides 1411-1827, and the rest of the sequence is a linking sequence between domains, such as figure 1 shown. The Cry30Fa1 domain I is amino acids 201-302 of the Cry30Fa1 protein, and the cor...

Embodiment 2

[0120] Embodiment 2, novel insecticidal protein gene HY131c anti-insect biological activity assay

[0121] The source of tested insects: rice brown planthopper (Nilapavata lugens ) was provided by Lai Fengxiang's laboratory, China Rice Research Institute.

[0122] Samples for testing:

[0123] The anti-N. lugens protein HY131c prepared in Example 1 is a prokaryotic expression and purified soluble protein. The concentration and yield are shown on the corresponding protein storage tube, sent on dry ice, and stored at -80°C;

[0124] Cry30Fa1 protein: the prokaryotic expression method in Example 1 was used to prepare Cry30Fa1 protein, and its amino acid sequence was sequence 4 in the sequence listing. Since the expression level of Cry30Fa1 protein was too low for bioassay, the Cry30Fa1 protein was truncated to increase its expression level. Under the premise of not affecting the three functional domains, amino acids 1 to 49 of the Cry30Fa1 protein were deleted, and the new pr...

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Abstract

The invention discloses insecticidal protein HY131c capable of resisting brown plant-hopper as well as an encoding gene and application thereof. The protein provided by the invention comprises structural domain I of Cry1Ac, structural domain II of Cry30Fa1 and structure domain III of Cry1Ac; and the protein is obtained by replacing the structural domain II in Cry1Ac protein with the structural domain II of Cry30Fa1 protein. Proved by experiment of the invention, the novel insecticidal protein provided by the invention has high toxicity to brown plant-hopper, thus providing a new strategy for resisting brown plant-hopper for rice, and having wide application prospect.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an insecticidal protein against rice brown planthopper, its coding gene and its application. Background technique [0002] Rice is an important food crop in the world, but rice pests and diseases seriously affect the production of rice. With the promotion and planting of Bt protein rice strains such as Cry1Ab and Cry1Ac, it is expected to effectively control the harm of Lepidoptera and Coleoptera pests (Shu, Ye et al.2000, Breitler, Vassal et al.2004, Cohen, Chen et al. .2008). However, it also inevitably promotes the population and damage degree of non-target pest insects such as brown planthopper (Chen et al.2011). Therefore, it is imminent to create and develop new insecticidal genes that are highly virulent to pests such as brown planthopper. [0003] Bacillus thuringiensis (Bt for short) is an Agrobacterium that forms spores and produces parasporal crystals under adverse cond...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K19/00C12N15/62A01N47/44A01P7/04
CPCA01N47/44C07K14/325C07K2319/00
Inventor 朱祯刘哲铭陈书元
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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