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Fetal-origin metabolic syndrome animal model building method and application thereof

A technology for metabolic syndrome and animal models, applied in the field of animal model construction, can solve problems such as differences in pathogenesis, achieve good stability, strong repeatability, and simple modeling methods

Active Publication Date: 2018-03-09
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Korach-Andre et al. first reported that in fa / fa Zucker obese rats, the MetS characteristics of insulin resistance could appear after administration of dexamethasone on day 0, day 1 and day 11 after birth. In obese rats, the time of drug administration is after birth rather than during pregnancy, so it can only reflect the "second hit" of the environment or drugs after birth, and there is a big difference in the pathogenesis of fetal-derived MetS

Method used

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  • Fetal-origin metabolic syndrome animal model building method and application thereof
  • Fetal-origin metabolic syndrome animal model building method and application thereof
  • Fetal-origin metabolic syndrome animal model building method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] [Example 1] Construction of animal model of fetal metabolic syndrome of the present invention

[0028] 1. Experimental animals:

[0029] SPF grade healthy Wistar / SD rats or Kunming mice were purchased from Hubei Provincial Center for Disease Control and Prevention, animal license number: SCXK (E) 2008-2010. This study was approved by the Ethics Committee of Wuhan University Medical Center, and was carried out in strict accordance with the relevant treatment guidelines of international laboratory animal protection certification and evaluation institutions.

[0030] The experimental animals were kept in a barrier environment with a temperature of 22-25° C. and a humidity of 50%, with 12 hours of day and night alternation.

[0031] 2. Experimental method:

[0032] 10 male Wistar rats (body weight 260-300g), and 20 female Wistar rats (body weight 200-240g). Free to drink and eat, after 7 days of adaptive feeding, according to male: female = 1:2 cage, vaginal smear in the...

Embodiment 2

[0067] [Example 2] Using the model of the present invention to screen environmental interferents or drugs for fetal metabolic syndrome

[0068] 1. The fetal metabolic syndrome model established according to the method of Example 1;

[0069] 2. Applying candidate environmental disruptors or drugs to animal models;

[0070] 3. Observe the influence of candidate environmental disruptors or drugs on various indicators of fetal metabolic syndrome, and evaluate potential environmental disruptors or drugs for fetal metabolic syndrome.

[0071] In summary, the modeling method of the present invention is treated with dexamethasone during pregnancy, and the offspring are routinely raised up to 28 weeks after birth. The experimental group has typical characteristics similar to glucose and lipid metabolism disorders and human metabolic syndrome, which is to establish fetal-derived An effective method for metabolic syndrome can be used to guide clinical rational drug use during pregnancy,...

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Abstract

The invention relates to a fetal-origin metabolic syndrome animal model building method and application thereof. A fetal-origin metabolic syndrome animal model is built by performing subcutaneous treatment of 0.1-2.0 mg / kg of hexamethasone per day on rodents 9-20 days after pregnancy and normally breeding baby rodents until 28-week-old and can present typical characteristics similar to those of metabolic syndromes of human. The built fetal-origin metabolic syndrome animal model is novel, reliable, simple and convenient and is significant to guiding reasonable clinical drug use during pregnancy, exploring action mechanism and molecular targets of fetal-origin metabolic syndrome, screening intrauterine environment disturbing chemicals and drugs and the like.

Description

technical field [0001] The invention relates to the technical field of animal model construction, in particular to a method for constructing an animal model of fetal metabolic syndrome. Background technique [0002] Metabolic syndrome (MetS) is a complex state of metabolic abnormalities such as abdominal obesity, dyslipidemia, hyperglycemia, and hypertension, which can increase the risk of type 2 diabetes, cardiovascular disease, and atherosclerosis. Insulin resistance is the main thread running through a variety of metabolic-related diseases, and is the common pathophysiological basis for the occurrence of MetS in adults. Between 20% and 25% of adults worldwide have MetS, and these individuals are five times more likely to develop type 2 diabetes than normal. In 2009, the International Diabetes Federation (IDF) and the American Heart Association / National Heart, Lung, and Blood Institute (AHA / NHLBI) defined MetS as abdominal obesity, hypertriglyceride (triglyceride, TG) -d...

Claims

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Application Information

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IPC IPC(8): A61K31/573A61P3/00A61P15/00
CPCA61K31/573
Inventor 汪晖徐丹郭喻敖英寇皓何波
Owner WUHAN UNIV
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