Method for detecting MMP-9 through graphene oxide fluorescent sensor

Abstract

The invention relates to a method for detecting MMP-9 through a graphene oxide fluorescent sensor and belongs to the technical field of biological detection. The method comprises carrying out hybridization on a dipeptide DNA complex containing a middle MMP-9 cleavage site and complementary molecules at two ends of a template DNA to obtain a DNA double-strand containing a middle neck loop structure, wherein the MMP-9 can be used for digestion at the dipeptide site and the neck loop DNA extends to form a single-stranded DNA fragment, mixing the fluorescently labeled single-stranded DNA and graphene oxide to cause DNA fluorescence quenching, and mixing the fluorescence quenching system and the DNA compound system subjected to digestion so that the fluorescently labeled DNA and the extending single-stranded DNA are complementary and hybridized to form a double-stranded DNA structure and the quenched fluorescence is recovered. According to a corresponding relationship of the MMP-9 concentration and the fluorescence intensity, MMP-9 can be detected. The method is easy to operate, has high sensitivity and good specificity and provides a feasible method for actual sample detection.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a method for detecting MMP-9 with a graphene oxide-based fluorescence sensor. Background technique [0002] Matrix metalloproteinases (MMPs) are a class of Ca-requiring 2+ , Zn 2+ As a large family of cofactors, such metal ions have degradative effects on various protein components of the extracellular matrix, and can exert physiological and pathological functions by regulating the degradation of extracellular matrix components, and are related to embryonic development, tissue differentiation, cell It plays a key role in tumor invasion and metastasis, and its role in tumor invasion and metastasis has attracted increasing attention. Matrix metalloproteinase-9 (MMP-9) is one of the members of matrix metalloproteinases. It is a metalloproteinase with a molecular weight of 92kDa secreted by most human cancer cells. MMP-9 has the activity of degrading type I...

AI Technical Summary

Problems solved by technology

[0005] Technical problems to be solved: The traditional MMP-9 immunological detection method needs to rely on the preparation of highly sensitive and specific antibodies. The preparation of antibodies requires professional operators to carry out a long-term preparation process. At the same time, the prepared antibodies are susceptible to environmental temperature, The influence of pH affects the sensitivity and stability of MMP-9 detection

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