Method for detecting MMP-9 through graphene oxide fluorescent sensor

A fluorescent sensor, MMP-9 technology, applied in fluorescence/phosphorescence, instruments, measuring devices, etc., can solve problems affecting the sensitivity and stability of MMP-9

Active Publication Date: 2018-03-13
浙江金果知识产权有限公司
View PDF5 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Technical problems to be solved: The traditional MMP-9 immunological detection method needs to rely on the preparation of highly sensitive and specific antibodies. The preparation of antibodies requires professional operators to carry out a long-term preparation process. At the same time, the prepared antibodies are susceptible to environmental temperature, The influence of pH affects the sensitivity and stability of MMP-9 detection

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting MMP-9 through graphene oxide fluorescent sensor
  • Method for detecting MMP-9 through graphene oxide fluorescent sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method for detecting MMP-9 based on a graphene oxide fluorescent sensor, comprising the steps of:

[0029] (1) Synthesis of graphene oxide

[0030] Weigh 12g of K 2 S 2 o 2 and 12g of P 2 o 5 Add it to 30mL concentrated sulfuric acid solution, place it on the heating reactor for heating, when the temperature reaches 80°C, add 5g graphene into the concentrated sulfuric acid solution for reaction, stop heating when the color of the solution turns dark blue, and put the solution Cool to room temperature; then dilute the solution 10 times and filter it with a 0.22 μm filter membrane to remove large precipitates and collect the filtrate, then dry it overnight at 80°C; place 50 mL of concentrated sulfuric acid in an ice bath Conditions of cooling, followed by adding 2g of the previously treated graphene oxide, then adding 10g of KMnO 4 , after stirring the reaction in an ice bath for 20min, add 2g of NaNO 3 , stirred at room temperature for 2 h, then added 500 mL of ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method for detecting MMP-9 through a graphene oxide fluorescent sensor and belongs to the technical field of biological detection. The method comprises carrying out hybridization on a dipeptide DNA complex containing a middle MMP-9 cleavage site and complementary molecules at two ends of a template DNA to obtain a DNA double-strand containing a middle neck loop structure, wherein the MMP-9 can be used for digestion at the dipeptide site and the neck loop DNA extends to form a single-stranded DNA fragment, mixing the fluorescently labeled single-stranded DNA and graphene oxide to cause DNA fluorescence quenching, and mixing the fluorescence quenching system and the DNA compound system subjected to digestion so that the fluorescently labeled DNA and the extending single-stranded DNA are complementary and hybridized to form a double-stranded DNA structure and the quenched fluorescence is recovered. According to a corresponding relationship of the MMP-9 concentration and the fluorescence intensity, MMP-9 can be detected. The method is easy to operate, has high sensitivity and good specificity and provides a feasible method for actual sample detection.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a method for detecting MMP-9 with a graphene oxide-based fluorescence sensor. Background technique [0002] Matrix metalloproteinases (MMPs) are a class of Ca-requiring 2+ , Zn 2+ As a large family of cofactors, such metal ions have degradative effects on various protein components of the extracellular matrix, and can exert physiological and pathological functions by regulating the degradation of extracellular matrix components, and are related to embryonic development, tissue differentiation, cell It plays a key role in tumor invasion and metastasis, and its role in tumor invasion and metastasis has attracted increasing attention. Matrix metalloproteinase-9 (MMP-9) is one of the members of matrix metalloproteinases. It is a metalloproteinase with a molecular weight of 92kDa secreted by most human cancer cells. MMP-9 has the activity of degrading type I...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N33/533
CPCG01N21/6428G01N33/533G01N2021/6432
Inventor 杨蕾
Owner 浙江金果知识产权有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap