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Antipyretic and antiviral active part of a compound traditional Chinese medicine and its preparation method and application

An active part, the technology of Reduning, which is applied to the antipyretic and antiviral active part of the traditional Chinese medicine compound Reduning injection and its preparation field, can solve problems such as potential safety hazards

Active Publication Date: 2021-02-09
JIANGSU KANION PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Reduning Injection's unclear active ingredients for specific diseases have brought certain hidden dangers to safety. Since the research of the existing technology is limited to the evaluation of the overall efficacy of "Reduning Injection", therefore, It is necessary to do further research on Reduning Injection to find out the active site / ingredients that can exert specific medicinal effects

Method used

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  • Antipyretic and antiviral active part of a compound traditional Chinese medicine and its preparation method and application
  • Antipyretic and antiviral active part of a compound traditional Chinese medicine and its preparation method and application
  • Antipyretic and antiviral active part of a compound traditional Chinese medicine and its preparation method and application

Examples

Experimental program
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Effect test

preparation example 1

[0026] Take the finished product of Reduning Injection and dry it under pressure to obtain 1 kg of the finished product concentrate, suspend it in 7.5 L of water, and separate it through HP-20 macroporous adsorption resin column chromatography, and then add water, 20%, 30%, 60%, 75% , eluted with 100% ethanol, collected 75%-100% ethanol eluate, concentrated under reduced pressure to no alcohol smell, and obtained 108 g of antipyretic and antiviral active fractions after freeze-drying. Wherein, in terms of mass percentage, the geniposide in the active part is 4.5%, the isochlorogenic acid B is 14.0%, the isochlorogenic acid A is 18.8%, and the isochlorogenic acid C is 32.5%.

preparation example 2

[0028] Take the finished product of Reduning Injection and dry it under pressure to obtain 1 kg of the finished concentrated solution, suspend it in 7.5 L of water, separate it through AB-8 macroporous adsorption resin column chromatography, and then wash it with water, 20%, 30%, 60%, 75% , eluted with 100% ethanol, collected 75%-100% ethanol eluate, concentrated under reduced pressure to no alcohol smell, and obtained 120 g of antipyretic and antiviral active fractions after freeze-drying. Wherein, in terms of mass percentage, the geniposide in the active part is 5.2%, the isochlorogenic acid B is 15.0%, the isochlorogenic acid A is 16.0%, and the isochlorogenic acid C is 28.4%.

preparation example 3

[0030] Take the finished product of Reduning Injection and dry it under pressure to obtain 1 kg of the finished concentrated solution, suspend it in 7.5 L of water, and separate it by chromatography on a DM301 macroporous adsorption resin column. % ethanol for elution, collect 75%-100% ethanol eluate, concentrate under reduced pressure until there is no alcohol smell, and freeze-dry to obtain 112 g of antiviral active fraction. Among them, in terms of mass percentage, the geniposide in the active part is 2.0%, the isochlorogenic acid B is 12.2%, the isochlorogenic acid A is 15.7%, and the isochlorogenic acid C is 27.8%.

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Abstract

The invention relates to a preparation method of the antipyretic and antiviral active part of the traditional Chinese medicine compound Reduning injection and its application in the preparation of antiviral medicine or antipyretic medicine. The preparation method disclosed by the invention adopts macroporous adsorption resin column chromatographic separation technology; and uses various chemical component separation methods and modern spectroscopic analysis means to separate and identify the chemical components of the active part. The active part in the present invention can significantly prolong the survival time of the stress mice infected with the influenza A (H1N1) FMI strain in vivo, reduce the death rate of the mice, and have a good antipyretic effect on the fever of the endotoxin shock mice induced by LPS. Therefore, Reduning injection and its active parts have good antipyretic and antiviral effects.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicines, in particular to an antipyretic and antiviral active part of a traditional Chinese medicine compound Reduning injection, a preparation method and application thereof. Background technique [0002] Under normal circumstances, the body's heat production and heat dissipation maintain a dynamic balance. Fever occurs when heat production increases or heat dissipation decreases due to various reasons. When various pathogens invade and other inflammatory stimuli, monocytes and macrophages release interleukin-1 (IL-1), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interfering v. -γ (IFN-γ) and other inflammatory factors act on the perioptic organs near the optic chiasm, activate phospholipase A2 (PLA2), and produce high levels of prostaglandin E2 (PGE2) through the cyclooxygenase (COX) pathway, stimulating Neurons in the anterior hypothalamus and brainstem responsible ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7048A61K31/216A61P29/00A61P31/16A61P31/14A61K31/7056A61K31/573
CPCA61K31/216A61K31/573A61K31/7048A61K31/7056A61K2300/00
Inventor 萧伟李海波葛雯房卉胡晗绯杨彪黄文哲王振中
Owner JIANGSU KANION PHARMA CO LTD
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