Micro-fluidic chip-based gene detection system and detection method thereof

Abstract

The invention relates to a micro-fluidic chip-based gene detection system and a detection method thereof. The detection system comprises a shell, a micro-fluidic chip, an optical device and a rotatingdevice. The micro-fluidic chip is arranged in an accommodating cavity, the micro-fluidic chip is provided with sample adding cells, sample separating elements and reaction elements, and every reaction element comprises a plurality of reaction cells loaded with various detection agents. The detection system has the advantages of realization of simultaneous detection of various pathogenic microorganisms through one-time sample loading, simple sample treatment steps, high detection efficiency, easiness in control of the sample adding volume, realization of the volumes of samples-to-be-detected entering all the reaction cells being same to each other, accurate detection result and automatic detection process, and meets the application requirements of high-efficiency and rapid detection and checking of a lot of pathogenic microorganisms in the port health quarantine.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a microfluidic chip-based gene detection system and detection method. Background technique [0002] Most of the highly pathogenic pathogenic microorganisms have the characteristics of strong infectivity, fast transmission, short incubation period and acute onset. threaten. At present, some highly pathogenic pathogenic microorganisms have crossed the barrier between species, and irregular outbreaks in humans have become an increasingly common phenomenon. Due to the complexity and mobility of port health and quarantine objects, and the unknown and variability of potentially highly pathogenic pathogenic microorganisms, it is very necessary to quickly detect highly pathogenic pathogenic microorganisms. [0003] Traditional detection methods mainly include direct smear microscopy, isolation and culture, etc. However, microscopic inspection methods relying on in vitro c...

AI Technical Summary

Problems solved by technology

However, the problem with the traditional immunoassay technology is that it is difficult to detect the window period of pathogenic microorganism infection. Even if the infected person has been infected with the virus, due to the small number of virus copies and the low abundance of virus antibodies, the results of checking the virus antibody are often missed diagnosis

Real-time fluorescence quantitative PCR technology is currently the mainstream method for the detection of nucleic acid molecules of pathogenic microorganisms. This method has strong specificity, but only one pathogenic target can be detected in one

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