An orange algae artificial turf and its application in coupling ammonium nitrogen wastewater treatment and carotenoid production

A technology of artificial turf and orange algae, applied in the biological field, can solve the problems of low cell adhesion strength of algal biofilm, easy detachment of cells from the attached matrix, and reduced pollutant removal rate, etc., and achieves easy popularization, simple method and preparation method. simple effect

Active Publication Date: 2020-08-11
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Unfortunately, the thickness of the biofilm formed by these methods is small, usually only 90-100 μm (a preparation method of algae biofilm, CN106011122A), and the number of cells is less and reduces the pollutant removal rate
On the other hand, the above-mentioned method forms the cell attachment strength of algae biofilm low, and cell is easy to break away from attachment matrix, needs the precultivation of long time for forming stable algae biofilm

Method used

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  • An orange algae artificial turf and its application in coupling ammonium nitrogen wastewater treatment and carotenoid production
  • An orange algae artificial turf and its application in coupling ammonium nitrogen wastewater treatment and carotenoid production
  • An orange algae artificial turf and its application in coupling ammonium nitrogen wastewater treatment and carotenoid production

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preparation example Construction

[0039] An orange algae artificial turf, the preparation method comprising: (1) breaking the orange algae into segmented algae filaments; (2) mixing the algae filaments with a liquid medium and a thickener; (3) mixing the mixed algae The uniform solution is sprayed on the surface of the matrix to form an algae film; (4) the algae film is cultivated for 5-10 days in suitable light intensity, temperature, ambient humidity and air enriched with carbon dioxide to form artificial turf with erect algae filaments.

[0040] Preferably, the orange algae is selected from strains of the family Chrysophyceae of the order Chlorophyceae Citrus, preferably selected from the strains of the genus Citrus and the genus Cephalosporin.

[0041] More preferably, the orange algae is selected from arboreal orange algae, golden orange algae, and Cephalosporium.

[0042] Preferably, the segmented algae filaments have a length in the range of 50-500 microns.

[0043] Preferably, the thickener is selecte...

Embodiment 1

[0050] Cultivate the golden orange algae to the logarithmic phase by liquid suspension, collect the plants and use a high-speed dispersing homogenizer to shear and disperse them. After running at a low speed for 1 minute, the length of the algae filaments in the mixed solution is in the range of 50-500 μm; then to the algae Add 50 times the volume of BBM medium and 0.1wt% xanthan gum to the silk and mix well, use a watering can to spray the mixed solution on the surface of the filter cloth to form an algae film; further, place the algae film at 30 μmol / m 2 Under the light intensity of / s and the temperature of 25°C, spray an appropriate amount of water to keep the algae film in a moist state, cultivate it for 10 days, and form artificial turf with upright algae filaments, and the thickness of the biofilm reaches 7.5mm.

Embodiment 2

[0052] Collect Cephaleuros plants, use a high-speed dispersing homogenizer to shear and disperse the plants, and obtain the length of algae filaments in the mixed solution in the range of 50-500 μm; add a certain volume of BBM medium to make the final concentration of algae filaments reach about 1g / L, add 0.1% sodium alginate and mix well, then sprinkle on the surface of the thin layer of sponge until the obvious green algae film is seen; then hang the sponge in a glass chamber, and enter the liquid BBM culture from the top of the sponge Base, and air containing 1% carbon dioxide was introduced into the chamber to provide 30μmol / m 2 / s light culture for 7 days, the length of upright algae filaments on the surface of the sponge reaches 10mm, and the preparation of orange algae artificial turf is completed.

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Abstract

The invention provides trentepohlia artificial grass and application thereof on coupling with ammonia nitrogen sewage treatment and carotenoid production. A large trentepohlia plant body is cut into fragment-shaped algal filaments, the fragment-shaped algal filaments are thickened, and then are inoculated into films so as to prepare the trentepohlia artificial grass; and the artificial grass is coupled with ammonia nitrogen sewage treatment and carotenoid production. Compared with the prior art, the trentepohlia artificial grass has the advantages that a selected trentepohlia film-forming method is simple, film-forming efficiency is high, and after being formed, the trentepohlia artificial grass is attached firmly, has quite high water impact resistance, and can grow in a water submergingor semi-submerging state. The algal filaments in the prepared trentepohlia artificial grass are in the shape of a stereo net which is upright mainly, the thickness reaches 10 mm, and the specific surface area of a whole biological film is large. The selected trentepohlia has efficient removal rate on ammonium nitrogen, has ability of resisting high-concentration ammonium nitrogen and a strong acidenvironment, and even can directly treat livestock and poultry breeding sewage and sanitary sewage. Steps are simple, and the trentepohlia artificial grass is convenient to operate and high in practicality.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to an orange algae artificial turf and its application coupled with ammonium nitrogen sewage treatment and carotenoid production. Background technique [0002] Microalgal cells are rich in high value-added components such as oil, protein, carbohydrates and natural pigments, and have broad application prospects in the fields of food, feed, medicine and biofuels. Microalgae need to absorb a large amount of nitrogen, phosphorus and carbon dioxide during the growth process, so they are also valuable in sewage treatment and carbon dioxide emission reduction. [0003] During the production and utilization of microalgae, harvesting microalgal biomass is a necessary process for recovering high value-added components and separating polluting components. At present, in the process of liquid suspension culture, algae biomass generally only accounts for 0.05%-1% of the total cu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P23/00C12N1/12C02F3/32C02F101/16C12R1/89
CPCC02F3/322C02F2101/16C12N1/12C12P23/00
Inventor 彭宇陈林刘天中汪辉王俊峰张维程文涛高莉丽
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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