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A Fast and Accurate Method for Dissecting Spider Intestines
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A spider and intestinal technology, used in autopsy, medical science, diagnosis, etc., can solve problems such as damage, intestinal rupture, and difficult identification, and achieve the effect of reducing pollution, ensuring integrity, and shortening dissection time.
Active Publication Date: 2020-03-10
HUBEI UNIV
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However, common spiders are small in size, with a body length of about 2-10 mm. Because the intestines are slender and fragile, they are not easy to identify and are easy to break. It is difficult to find the intestines accurately by conventional methods, which not only wastes a lot of time, but also Highly prone to intestinal rupture or damage
[0005] After searching the existing literature at home and abroad, there is no report on a fast and accurate spider intestinal dissection method
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Embodiment 1
[0022] Example 1: Anatomy of the intestine of a male Araneus karinii
[0023] 1. Materials and reagents: sterile petri dishes, 75% alcohol.
[0026] (1) Take a male Araneus karinii that died naturally in the laboratory, disinfect the surface of the spider with 75% alcohol on an ultra-clean workbench for 5 minutes, wipe the body surface with sterilized absorbent cotton, and then use 75% Rinse with alcohol 3 times.
[0027] (2) Put the spider in a sterile petri dish and pour 75% alcohol to submerge the spider.
[0028] (3) With the back of the spider facing up and the belly facing down, the left hand uses the tweezers to clamp the outer skin 0.5-1mm behind the back abdominal stalk, and the right hand uses the tweezers to tear the outer skin at the clamped place.
[0029] (4) After tearing open the outer epidermis, the heart wrapped by muscle is exposed. The muscle tissue on both sides of the heart...
Embodiment 2
[0032] Example 2: Anatomy of the intestine of a female Araneus karinii
[0033] 1. Materials and reagents: sterile petri dishes, 75% alcohol, physiological saline.
[0034] 2. Instruments and tools: microscope, pointed tweezers.
[0035] 3. Operation steps:
[0036] (1) Take a female Araneus karinii that died naturally in the laboratory, disinfect the surface of the spider with 75% alcohol on a clean workbench for 5 minutes, wipe the body surface with sterilized absorbent cotton, and then use 75% alcohol Rinse 3 times.
[0037] (2) Put the spider in a sterile petri dish and pour saline to submerge the spider.
[0038] (3) With the back of the spider facing up and the abdomen facing down, the left hand uses the tweezers to clamp the outer skin 0.5-1mm behind the back abdominal stalk, and the right hand uses the tweezers to tear the outer skin at the clamped place. The female spider has a larger abdomen And it's rounded, so tear it apart slowly, otherwise the contents will overflow.
[00...
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Abstract
The invention provides a spider intestinal tract dissection method, and belongs to the field of arthropodanatomy. According to the method, dissection is started from back, heart and feces sac are taken as a breakthrough, and the intestinal tract position can be quickly and accurately found; meanwhile, the integrality of the intestinal tract can be guaranteed to the maximum extent. The whole dissection process only needs 5-10 minutes, the dissection time is greatly shortened, the pollution and the waste of spider materials are reduced, a favorable experimental material basis is provided for the follow-up researches of pest prevention and control, spider intestinal tract microorganism and the like.
Description
Technical field [0001] The invention belongs to the technical field of arthropodanatomy, and specifically relates to a fast and accurate method for dissecting spider intestines. Background technique [0002] Spiders belong to the phylum Arthropods. The body is divided into a head, chest and abdomen. The abdomen is not segmented. The abdomen stem evolved from the first abdominal segment. The abdomen contains the digestive system, heart, reproductive organs and silk glands. [0003] Spiders are important natural enemies of many pests. With the increase of genetically modified plants, pests also increase. Modern biotechnology can combine spiders with pesticide control to increase the effectiveness of pest control. The food of spiders is closely related to these pests. Spiders are predatory natural enemies. Through the detection of intestinal contents, it is possible to understand the types of pests preyed by different kinds of spiders. It is necessary to study the related problems of...
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