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Ovarian cancer Annexin A3 detection kit

A detection kit and ovarian cancer technology, applied in the field of tumor detection, can solve the problems of poor repeatability, poor sensitivity, unstable experimental data, etc., and achieve the effects of alleviating storage and transportation, strong accuracy, and high sensitivity

Inactive Publication Date: 2018-03-23
BEIJING YINUO YISHENG TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the sample size is large, although the Annexin A3ELISA Quantitative Detection Kit has achieved high-throughput detection, after strict quality control, the kit showed outstanding problems of poor sensitivity and poor repeatability, resulting in Instability of experimental data

Method used

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  • Ovarian cancer Annexin A3 detection kit
  • Ovarian cancer Annexin A3 detection kit
  • Ovarian cancer Annexin A3 detection kit

Examples

Experimental program
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Embodiment Construction

[0030] The preparation, use and effect of the ovarian cancer Annexin A3 detection kit will be described in further detail below in conjunction with the accompanying drawings.

[0031] Preparation of Ovarian Cancer Annexin A3 Detection Kit:

[0032] 1. Main reagents and materials

[0033] Human Annexin A3 recombinant protein, horseradish peroxidase, bovine serum albumin, horseradish peroxidase-labeled goat anti-mouse IgG, chemiluminescent microplate strips;

[0034] 2. Preparation of main reagents (both take 1000mL as an example)

[0035] (1) Standard diluent

[0036] Weigh 10g BSA and add it into 1000M1 0.02mol / L pH7.4 phosphate buffer solution to dissolve, add 1mL Proclin300, and mix well;

[0037] (2) Blocking solution

[0038] Weigh 5g BSA, add 1000mL 0.02mol / L Ph7.4 phosphate buffer solution to dissolve, add 1mLProclin300, mix well;

[0039] (3) Enzyme detection antibody diluent

[0040] 200mL calf serum plus 800mL 0.02mol / L pH7.4 phosphate buffer plus 1mL Proclin300...

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Abstract

An ovarian cancer Annexin A3 detection kit includes a pore plate coated with an Annexin A3 capture antibody, Annexin A3 standard substances S0-S5, quality control substances respectively with the concentrations of 1.6 ng / mL and 0.8 ng / mL, a horseradish peroxidase labeled detection antibody, a chemiluminescence substrate liquid A, a chemiluminescence substrate liquid B, and a concentrated paint-washing liquid. By adopting the detection kit, no matter whether the sample amount is large, the ovarian cancer Annexin A3 detection kit can guarantee relatively strong sensitivity and relatively high accuracy.

Description

technical field [0001] The invention relates to the field of tumor detection, in particular to an ovarian cancer Annexin A3 detection kit. Background technique [0002] Ovarian cancer is the female reproductive system malignant tumor with the highest mortality rate, and the resistance of tumor cells to chemotherapy drugs is the main obstacle to clinical treatment. Early detection of tumor drug resistance by using cobalt-based drug resistance marker proteins will help clinically adjust chemotherapy regimens in time and improve patient prognosis. [0003] Clinical evaluation criteria for platinum-based drugs in patients with ovarian cancer include: tumor progression during chemotherapy, tumor recurrence within one month of stopping chemotherapy, and no change in measurable tumors as the best response to first-line chemotherapy. The biggest feature of this standard is that the patient's sensitivity to chemotherapy drugs cannot be judged until the chemotherapy has progressed to...

Claims

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Application Information

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IPC IPC(8): G01N21/76G01N33/574
Inventor 刘佐雄
Owner BEIJING YINUO YISHENG TECH CO LTD
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