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Penis cervi active peptide and preparation method thereof

A technology of active peptide and deer whip, which is applied in the biological field, can solve the problem of lack of preparation method of deer whip peptide, and achieve the effect of improving health care function

Inactive Publication Date: 2018-03-30
林峰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] With the development of science and technology, the application of protein engineering technology, the research and product development of bioactive peptides and their protein hydrolyzates using enzymatic hydrolysis technology has become a hot spot and mainstream, but there is no preparation method for the production of deer whip peptide in industry.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A preparation method of deer whip active peptide, comprising the following steps:

[0036] A. Pretreat the deer penis, carry out ultrafine grinding, pass through 400 mesh, add water to mix, adjust pH 8-9 to slightly alkalinize, soak for 20 hours. Mechanical stirring, centrifugation of the material and liquid in the reaction tank, and collecting the supernatant;

[0037] B. Add acetic acid to the supernatant to precipitate protein. Adjust the pH value of the supernatant to 4-6, mechanically stir, centrifuge, collect the precipitate, and the precipitate is deer whip protein;

[0038] C. Redissolve the precipitate with water, heat to 90 degrees to denature the protein, and then perform high-pressure homogenization;

[0039] D. Adjust the pH of the above solution to 7-8, add a special enzyme preparation (flavor protease: trypsin: neutral protease is 1.5: 1.5: 1), enzymolysis, enzymolysis temperature is 50 ° C, and enzymolysis time is 2h;

[0040] E. After the enzymatic h...

Embodiment 2

[0052] A preparation method of deer whip active peptide, comprising the following steps:

[0053] A. Pre-treat the dried deer penis, carry out superfine grinding, pass through 400 meshes, add water to mix, adjust the pH to 8-9 for micro-alkaline treatment, and soak for 20 hours. Mechanical stirring, centrifugation of the material and liquid in the reaction tank, and collecting the supernatant;

[0054] B. Add acetic acid to the supernatant to precipitate protein. Adjust the pH value of the supernatant to 4-6, mechanically stir, centrifuge, collect the precipitate, and the precipitate is deer whip protein;

[0055] C. Redissolve the precipitate with water, heat to 90 degrees to denature the protein, and then perform high-pressure homogenization;

[0056] D. Adjust the pH of the above solution to 7-8, add a special enzyme preparation (flavor protease: trypsin: neutral protease is 1.4: 1.6: 1.2), enzymolysis, enzymolysis temperature is 50 ° C, and enzymolysis time is 2h;

[00...

Embodiment 3

[0069] A preparation method of deer whip active peptide, comprising the following steps:

[0070] A. The dried deer penis is pretreated, ultrafinely pulverized, passed through 400 mesh, mixed with water, adjusted to pH 8-9 for slightly alkaline treatment, and soaked for 22 hours. Mechanical stirring, centrifugation of the material and liquid in the reaction tank, and collecting the supernatant;

[0071] B. Add acetic acid to the supernatant to precipitate protein. Adjust the pH value of the supernatant to 4-6, mechanically stir, centrifuge, collect the precipitate, and the precipitate is deer whip protein;

[0072] C. Redissolve the precipitate with water, heat to 95 degrees to denature the protein, and then perform high-pressure homogenization;

[0073] D. Adjust the pH of the above solution to 7-8, add a special enzyme preparation (flavor protease: trypsin: neutral protease is 1.6: 1.6: 1.4), enzymolysis, the enzymolysis temperature is 55 ° C, and the enzymolysis time is 1...

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Abstract

The invention provides a method for preparing a penis cervi active peptide. According to the preparation method, penis cervi is utilized as a raw material to prepare a refined penis cervi active peptide and a total nutrient penis cervi active peptide; high-efficiency enzymolysis is performed by adopting the combination of enzyme preparations, and energy consumption and material consumption can bereduced; traditional Chinese medicinal materials fructus rubi and clove are creatively added in extraction and enzyme deactivation, so that the nutritional substances of penis cervi peptide can be further enriched; a specific filtration adjuvant combination is adopted in the separating process, so that the refining and separating capability is greatly reinforced; and finally, a medium-low temperature spray drying tower is adopted to protect thermosensitive active substances, and is applied to a peptide process for the first time. Furthermore, the preparation method is easily available in raw materials and simple in production process, so that the production cost can be greatly reduced. The mass content of the refined penis cervi active peptide is more than or equal to 80 percent, and the mass content of the total nutrient penis cervi active peptideis more than or equal to 30 percent.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a deer whip active peptide and a preparation method. Background technique [0002] Deer whip, the name of traditional Chinese medicine, also known as deer kidney and deer Chong. It is the external genitalia of the male sika deer or red deer of the deer family. Deer whip has many functions, such as promoting blood circulation and promoting lactation. Sweet in the mouth, sweet, warm in nature. Can invigorate the kidney and strengthen yang, benefit essence. It can be used to nourish yin and strengthen yang, and stimulate male sexual function; for sexual dysfunction, deer penis soaked in wine can be used to make deer penis tincture, and deer penis can be used to treat excessive urination in the elderly at night. Troublesome urination at night. The deer whip can be soaked in wine, made into medicinal food, or cooked, or boiled into ointment, or made into pills or powder. ...

Claims

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Application Information

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IPC IPC(8): A23J1/00A23J3/04A23J3/34A23L33/18A23L33/10
CPCA23J1/002A23J3/04A23J3/341A23L33/10A23L33/18
Inventor 林峰
Owner 林峰
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