Micro-fluidic chip as well as modifying method and application thereof to detection of quantity of food bacteria

A microfluidic chip and modification technology, applied in chemical instruments and methods, laboratory containers, instruments, etc., can solve the problems of high requirements for surface modification of microfluidic materials, achieve fast detection time and reduce infection risk Effect

Inactive Publication Date: 2018-03-30
GREENTOWN AGRI TESTING TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the equipment of this method is relatively cheap, in order to reduce the noise signal generated by other non-target particles in the food sample and the interference to the detection signal, this method requires high surface modification of microfluidic materials.

Method used

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  • Micro-fluidic chip as well as modifying method and application thereof to detection of quantity of food bacteria
  • Micro-fluidic chip as well as modifying method and application thereof to detection of quantity of food bacteria
  • Micro-fluidic chip as well as modifying method and application thereof to detection of quantity of food bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] A method for modifying a microfluidic chip, comprising the following steps:

[0049] (1) Put the microfluidic chip of polydimethylsiloxane (PDMS) into the plasma generator to react, the reaction conditions are: the pressure is 100mTorr, the power is 118W, and the reaction time is 10 seconds. The purpose is to put the –CH on the inner surface of the microfluidic channel 3 The group is transformed into a -OH group;

[0050] (2) After the reaction of the step (1) is completed, the mixed solution of APTMS (aminopropyltrimethoxysilane) and dehydrated alcohol is passed into the micro-flow pipeline to carry out silanization reaction, wherein the mass ratio of APTMS is 5%, after reacting for 30 seconds, air was blown to dry the remaining mixed solution in the microfluidic channel, and dried naturally at room temperature. This step can introduce amino groups on the surface of the microfluidic channel.

[0051] (3) After drying, the seventh-generation PAMAM dendrimer whose surf...

Embodiment 2

[0072] Using the same method as in Example 1, the only difference is that the grafted dendrimers are PAMAM G4 (the fourth generation PAMAM dendrimers). For the schematic diagram of the reaction, see figure 1 .

[0073] After the above-mentioned modification was completed, the Escherichia coli in the sample to be tested was detected by the same detection method as in Example 1, and the detection results are shown in Table 1.

Embodiment 3

[0075] Using the same method as in Example 1, the only difference is that the grafted dendrimers are PAMAM G9 (ninth generation PAMAM dendrimers).

[0076] After the above-mentioned modification was completed, the Escherichia coli in the sample to be tested was detected by the same detection method as in Example 1, and the detection results are shown in Table 1.

[0077] From Figure 5 It can be seen that the detection limit of grafted PAMAM G7 to Escherichia coli is 10 2cells / mL, the detection time is within 4 hours, and the detection limit of most existing optical microfluidic devices is 10 4 cells / mL, therefore, after the modification of Example 1 of the present invention, it can far exceed the existing detection equipment.

[0078] Wherein the detection performance of embodiment 1 of the present invention is higher (antibody modification detection limit is 10 4 ), the reason is that each G7 macromolecule can graft one antibody due to the larger volume of the antibody (a...

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Abstract

The invention discloses a microfluidic chip and its modification method and its application in detecting the number of food bacteria. The modification method includes the following steps: (1) transforming - The CH3 group is modified into an OH group; (2) after the step (1) reaction is completed, the aminosilane reagent is passed into the micro-flow channel to carry out the aminosilane reaction; (3) after drying, the The dendrimer macromolecules whose surface has been modified by -COOH are introduced into the micro-flow channel to carry out the coupling reaction; (4) after the step (3) reaction is completed, the bacteria to be tested are introduced into the micro-flow channel An aptamer solution with specific recognition performance and whose 5' end is modified by amino group, so that the aptamer is grafted onto the surface of the dendrimer. The invention modifies the microfluidic chip, can quickly detect the number of food bacteria, improves the detection efficiency, and the whole detection environment is independent and safe, preventing cross-contamination.

Description

technical field [0001] The invention belongs to the technical field of food detection, and in particular relates to a microfluidic chip, a modification method thereof and an application in detecting the number of food bacteria. Background technique [0002] In recent years, food-induced diseases have attracted more and more attention. Therefore, for the field of food hygiene inspection, fast and sensitive detection equipment is very important. Similarly, for food processing enterprises and food monitoring departments, if the number of target bacteria in food can be quickly detected, the monitoring of food quality can be improved, so as to effectively implement the protection of public food hygiene and safety. [0003] The traditional method of bacterial detection is the method of colony culture, which can accurately determine whether the harmful target bacteria in food exceed the standard, but the disadvantage of this method is that the waiting time for the result is too lo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N35/00B01L3/00
CPCG01N35/00029B01L3/502707B01L2200/10B01L2200/12G01N2035/00158
Inventor 郝星凯曹旭东王珍尤坚萍章舒褀
Owner GREENTOWN AGRI TESTING TECH CO LTD
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