Method for quickly screening pesticides, drugs and converting products in waste water at same time

Abstract

The invention discloses a method of quickly screening pesticides, drugs and converting products in waste water at the same time, belonging to the field of analytical chemistry. The method comprises the following steps: (a) carrying out analytic detection, namely analytically detecting a sample; (b) carrying out pesticide and drug identification, namely screening by adopting pesticide and drug database software to identify whether pesticides and drugs exist or not; and (c) carrying out identifying-non-targeted screening of the converting products, namely predicting the converting products of identified pesticides and drugs in the step (b) through software, carrying out secondary spectrogram analysis and compound screening on an index compound of pesticides and drugs to predict diagnostic ions, and analyzing and identifying the converting products by extracting corresponding chromatographic peaks on the compound comprising the diagnostic ions. According to the method, pesticides, drugs and converting products in waste water can be screened at the same time, and the screening speed is high, the range is wide and the accuracy is high.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and uses liquid chromatography-time-of-flight mass spectrometry technology to carry out a high-throughput identification and identification method for pesticides, drugs and their transformation products in wastewater. Background technique [0002] With the development of the economy, more and more pesticides and drugs are produced. These chemicals are widely used in daily life after production. Due to the strong polarity of pesticides and pharmaceutical compounds, they are easy to migrate through water bodies and enter natural water bodies. Therefore, the detection of pesticides and drugs in wastewater has always been the focus of research by personnel in this field. [0003] At present, domestic analysis and research on pesticide and drug compounds in wastewater is usually the direct standard sample quantification of the compound; because the standard sample is expensive, there are many types...

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Problems solved by technology

[0005] China Patent No. 201410028583.9, the application for which the date of authorization announcement is 2016.08.10 has announced a method for detecting enrofloxacin metabolites in sea cucumbers. The method includes sample extraction, purification, drawing of a standard curve, instrument conditions and Structural analysis of metabolites, which uses liquid chromatography-tandem mass spectrometry-ion trap technology to accurately determine the predicted metabolites using the pMRM method, using QTrap's unique MRM-IDA-EPI working mode, the MRM diagram of the corresponding metabolites can be obtained , and get its MS 2 Mass spectrogram; ten kinds of metabolites including ciprofloxacin can be identified, but the workflow of using the MRM-IDA-EPI mode in the method of this application is: respectively measure the sample solution to be tested and the blank control group, for Each set of pre-set ion pairs is compared to analyze the difference between the MRM extraction ion flow chromatogram of the sample solution and the blank control group, and the quasi-molecular ions, retention time and extraction ion pairs of metabolites are analyzed to find out the Enno The possible metabolites of Floxacin in sea cucumbers combined with MS 2 The EPI mass spectrogram of the EPI mass spectrogram, its fragment ions were analyzed by mass spectrometry, and finally the structure of the metabolite was deduced according to the molecular structure of enrofloxacin. Therefore, the method of using this application is relatively cumbersome to operate and can only be aimed at a single sample, such as It is not applicable for identification and screening of a large number of pesticides, drugs and metabolites present in wastewater

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