Improvement and application of oral cavity swab sample preservation solution

Abstract

The invention discloses improvement and application of an oral cavity swab sample preservation solution. The oral cavity swab sample preservation solution comprises 5-20mM of Tris, 10-50mM of EDTA, 0.1-0.5M of NaCl, 0.1-.05% of SDS and 0.1-2% of sodium hydroxymethylglycinate. The oral cavity swab sample preservation solution also comprises food preservatives nationally licensed to be used. The preservation temperature of the oral cavity swab sample preservation solution is about 37 DEG C, reagents used in a formula of the preservation solution are cheap and fine, and DNA detection rate and stability are improved, so that detection cost is reduced; the formula and production steps are simple and convenient, and the used reagents are good in stability, so that long-term storage of a productcan be facilitated; and the preservation solution adopts common preservatives in foods and daily necessities for inhibiting damage of bacteria on an oral cavity swab sample, the preservatives fully pass through related verification, use security is high, ingredients of the prepared oral cavity swab sample preservation solution can be compatible with a subsequent DNA extraction program, and subsequent DNA extraction is beneficial.

Description

technical field [0001] The invention relates to an oral swab sample preservation solution, in particular to the improvement and application of an oral swab sample preservation solution. Background technique [0002] Oral swabs are easy to use and are one of the most commonly used sampling consumables in DNA testing. Extracting high-quality DNA samples from well-sampled buccal swabs is the most important experimental basis for DNA testing. Therefore, how to store the samples stably and ensure that the DNA in the sampling swab is completely and adequately retained is a key issue. Generally, after the sampling is completed, a certain amount of preservation solution needs to be added to the preservation tube to ensure the stability of the DNA and prevent it from being degraded by saliva and enzymes in the environment. In our work, there are oral swabs from different customers. The effects of oral swab preservation solutions in different forms and compositions vary greatly. The...

AI Technical Summary

Problems solved by technology

In our work, there are oral swabs from different customers. The effects of oral swab preservation solutions in different forms and compositions vary greatly. The first difficulty to be overcome in the stable storage of samples collected from the oral cavity is to inhibit the impact of bacteria on the collection. Degradation of oral exfoliated cells

A second consideration is the protection of cell-free DNA