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Building method of mouse asthma model sensitized by humulus pollen

A construction method and pollen technology, applied in the direction of allergen antigen components, can solve the problems of mouse death, low airway reactivity, and insignificant serological changes, and achieve the effect of avoiding adverse consequences

Active Publication Date: 2018-04-17
PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in our experiments, it was found that the airway reactivity caused by intraperitoneal injection was low, the inflammatory response was weak, and the serological changes were not obvious, which could not simulate the typical characteristics of human asthma. And because of direct and strong stimulation to the airway, it is easy to cause acute lung injury in mice, resulting in death of mice

Method used

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  • Building method of mouse asthma model sensitized by humulus pollen
  • Building method of mouse asthma model sensitized by humulus pollen
  • Building method of mouse asthma model sensitized by humulus pollen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Example 1: Selection of anesthesia method during mouse sensitization

[0069] 1. Purpose

[0070] In the construction of this model, the mice need to be anesthetized before the sensitization operation in the sensitization process. Choosing an appropriate, safe and effective anesthesia method is the basis for experimental safety and data reliability. This part of the study is to compare the survival rate, anesthesia onset time and anesthesia maintenance time of mice after three different anesthesia methods (injection of pentobarbital sodium, inhalation of ether, and inhalation of isoflurane) in order to select sensitization The most suitable method of anesthesia.

[0071] 2. Materials and methods

[0072] 1) Main reagents

[0073] Diethyl ether Luoyang Wuhua Chemical Reagent Co., Ltd. China

[0074] Isoflurane Hebei Yipin Pharmaceutical Co., Ltd. China

[0075] Sodium Pentobarbital Sigma Corporation USA

[0076] 2) Main instruments

[0077] Universal Animal Anest...

Embodiment 2

[0108] Example 2: Evaluation of the effect of establishing a mouse asthma model by intraperitoneal injection sensitization + intranasal challenge 1. Purpose

[0109] Intraperitoneal injection sensitization + intranasal challenge is a commonly used method to establish a mouse asthma model, but whether this method is applicable to the establishment of a humulus pollen-sensitized mouse asthma model remains to be verified experimentally. This part of the study is to try to use the method of intraperitoneal sensitization + intranasal challenge to construct a mouse asthma model and evaluate the modeling effect.

[0110] 2. Experimental objects and materials

[0111] research object

[0112] 1) Animal preparation

[0113] Thirty 6-8-week-old female BALB / c mice (SPF grade), weighing 18-20 g, were provided by the Chinese Academy of Military Medical Sciences and raised in the animal room of Peking Union Medical College Hospital, free to eat and drink aseptically. Breeding environment...

Embodiment 3

[0169] Example 3 Comparing the effect of subcutaneous injection sensitization and intraperitoneal injection sensitization model construction

[0170] 1. Purpose

[0171] Both subcutaneous injection sensitization and intraperitoneal injection sensitization are commonly used sensitization methods. The operation of intraperitoneal sensitization is simpler, but some studies also suggest that subcutaneous sensitization can elicit a stronger immune response. The purpose of this part of the study is to determine a more suitable sensitization method for the construction of humulus pollen-sensitized asthma mouse models by comparing the effects of subcutaneous injection sensitization and intraperitoneal injection sensitization.

[0172] 2. Experimental objects and materials

[0173] 1) Animal preparation

[0174] 40 female BALB / c mice (SPF grade) aged 6-8 weeks, weighing 18-20 g, were provided by the Chinese Academy of Military Medical Sciences, raised in the animal room of Peking Un...

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Abstract

The invention provides a building method of mouse asthma model sensitized by humulus pollen. The building method includes following steps: 1, sensitizing: subcutaneously injecting 200ul of humulus pollen sensitizing liquid behind the neck of a BALB / c mouse on the first day, the eighth day and the fifteenth day respectively; 2, exciting: enabling the mouse to inhale 1ug / ul of humulus pollen proteinextract through atomization for 30min each day for 7 days consecutively. The humulus pollen protein extract is used to perform subcutaneous injection sensitizing and atomization exciting on the BALB / c mouse, and the mouse asthma model sensitized by the humulus pollen is built successfully. The model mouse can well simulate typical features of human asthma caused by humulus pollen sensitizing. Themodel is about to provide a good platform for people to study pathogenesis of human asthma caused by humulus pollen sensitizing and to explore new treatment methods.

Description

technical field [0001] The invention relates to a new method for constructing allergic asthma mouse model, that is, a method for constructing humulus pollen-sensitized mouse asthma model. Background technique [0002] Allergic asthma is a complex multifactorial disease caused by inhaled allergens, and its incidence is increasing worldwide. In my country, the annual herbaceous plant Humulus scandens is widely distributed throughout the country due to low requirements for growth conditions, and its pollen is an important cause of allergic asthma in summer and autumn. [0003] To study the pathogenesis of Humulus pollen-induced asthma and find more effective treatments, animal models are important tools. Mice are the most commonly used model research objects due to their lung structure, histology, and functional characteristics similar to humans, low feeding costs, and easy development of transgenic models. A typical allergic asthma mouse model should be able to simulate the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/36
CPCA61K39/36
Inventor 尹佳席广鹏孔瑞
Owner PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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