Method for quickly and accurately breeding maize inbred line by maize whole genomics technology

A whole-genome, inbred line technology, applied in the fields of botanical equipment and methods, plant genetic improvement, application, etc., can solve the problems of insufficient aggregation of comprehensive excellent traits, long years of breeding maize inbred lines, and heavy workload, etc. Achieve the effect of shortening the improved generation, speeding up the breeding speed, and expanding the genetic background

Pending Publication Date: 2018-04-20
WIN ALL HI TECH SEED CO LTD +3
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Problems solved by technology

[0005] The purpose of the present invention is to overcome the deficiencies in the prior art, and provides a method for quickly and accurately breeding corn inbred lines using the whole genome technology of corn, aiming at solving the problems of long years and work problems in the existing breeding of corn inbred lines. Problems such as large quantity and insufficient aggregation of comprehensive and excellent properties

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  • Method for quickly and accurately breeding maize inbred line by maize whole genomics technology
  • Method for quickly and accurately breeding maize inbred line by maize whole genomics technology

Examples

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Effect test

Embodiment 1

[0024] This example provides a kind of Huanghuaihai core inbred line "Zheng 58" with better plant type, shorter plant height, fertilizer and water tolerance, drought tolerance, lodging resistance and disease resistance, which is to be transformed by using the whole genome technology of corn. Breeding parents, rapid and accurate breeding of better new varieties of corn inbred lines, the technical roadmap is as follows figure 1 shown, including the following steps:

[0025] (1) Obtained from BC1F2 generation segregation population

[0026] The inbred line "Zheng 58" was used as the female parent, and the inbred line "ph6wc" with excellent field agronomic traits, high seed yield, density tolerance, and good quality was used as the male parent, and the female parent and the male parent were routinely sexually After crossing, backcross with the male parent once, and then self-cross the obtained BC1F1 generation again, and obtain BC1F2 generation segregation population, a total of ...

Embodiment 2

[0041] This example provides a method of using the whole genome technology of maize, using the inbred line "Chang 7-2" with good plant type, high temperature resistance, high combining ability, wide adaptability and multi-resistance as the parent to be transferred, to carry out rapid and accurate The method for breeding better new varieties of corn inbred lines, the technical roadmap is as follows: figure 2 shown, including the following steps:

[0042] (1) Acquisition of BC1F2 generation population

[0043] The inbred line "Chang 7-2" was used as the female parent, and the inbred line "ph4cv" with excellent agronomic traits, high seed production rate, and high quality was used as the male parent. After conventional sexual crossing between the female parent and the male parent, The BC1F1 generation was backcrossed once with the male parent, and the obtained BC1F1 generation was self-crossed again to obtain a segregation population of the BC1F2 generation, with a total of 958...

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Abstract

The invention discloses a method for quickly and accurately breeding a maize inbred line by a maize whole genomics technology, wherein the method combines the traditional breeding with a whole genomesequencing technology. Through field agronomic characteristic screening and genotype analyzing, an excellent individual plant with excellent trait genes of both parents and genetic background more close to a target parent is screened, finally the stable inbred line combined with excellent traits of both parents are bred through continuous selfing. Compared with the prior art, the method performs genetic background screening through a genetic typing technology of conventional selection and whole genome sequencing, the improvement generation of the inbred line is greatly shortened, the maize inbred line with excellent comprehensive characters can be accurately bred within only 2 to 3 years.

Description

technical field [0001] The invention relates to the technical field of corn breeding, in particular to a method for rapidly and accurately breeding corn inbred lines using the whole genome technology of corn. Background technique [0002] With the continuous deepening of marketization and the introduction of a large number of foreign excellent germplasm resources, the quality and quantity of corn varieties cultivated in my country have been greatly improved. Chinese corn breeders have successively introduced resources from abroad and bred a large number of excellent hybrids. Varieties, such as Yedan series, Nongda series, Zhengdan series, etc., have made great contributions to the increase of corn income and production in my country, but there are still some breakthrough corn varieties with excellent comprehensive characteristics. [0003] The breeding practice of corn hybrid varieties has proved that the excellent traits of the parents are one of the core factors to improve ...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 张从合韩斌黄学辉王慧陈金节蒋家月冯旗严志方玉王阿红黄艳玲
Owner WIN ALL HI TECH SEED CO LTD
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