Anti-aging composition and application thereof
An anti-aging and composition technology, applied in the field of daily necessities, to achieve good anti-aging effect
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Embodiment 1
[0062] Formulation composition according to table 1, table 2
[0063] Table 1
[0064]
Example 1
Example 2
Example 3
Example 4
Example 5
Example 6
first active ingredient
1%
2%
1.5%
1%
4%
3.75%
second active ingredient
0.5%
1%
1.5%
2%
2%
1.5%
[0065] Table 2
[0066]
Comparative Example 1
Comparative example 2
Comparative Example 3
Comparative example 4
Comparative Example 5
first active ingredient
2%
0
4%
0
0
second active ingredient
0
1%
0
2%
0
[0067] The auxiliary materials of above-mentioned embodiment 1-embodiment 5, comparative example 1-comparative example 5 are: xanthan gum 0.1%, carbomer 0.18%, betaine 3%, triethanolamine 0.2%, squalane 3%, tocopherol Phenyl acetate 1%, 1,3-propanediol 0.1%, caprylyl hydroxamic acid 0.8%, glyceryl caprylate 0.5%, 1,3-butanediol 0.1%, 1,2-pentanediol 0.1%, Maltodextr...
Embodiment 2
[0071] (1) Test the expression of matrix metalloproteinase-1 (MMP-1)
[0072] Young fibroblasts (cells selected from a 32-year-old adult woman in this example) and aging adult fibroblasts (cells selected from a 64-year-old adult woman in this example) were respectively inoculated into 48-well cell culture plates, Each hole contains 0.75*10 5 cells were starved for 24 h in serum-free medium. Starved cells were rinsed with PBS and treated with UV light. The test samples shown in Table 1 and Table 2 were then added within 48 hours. After 72h, the expression of isolated MMP-1 in the culture medium was measured using the MMP-1 kit. With blank control (comparative example 6) senile fibroblasts as MMP-1 expression 100%, measure other samples to be tested (embodiment 1-embodiment 6, comparative example 1-comparative example 5) relative to this comparative example 6 MMP-1 expression level.
[0073] The test results are shown in Table 3 (the concentration of Example 1-Example 6, Co...
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