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Lactobacillus plantarum as well as lactobacillus plantarum freeze-dried powder and preparation method thereof

A technology of Lactobacillus plantarum and freeze-dried powder, applied in the biological field, can solve the problems of low number of viable bacteria, easy inactivation of bacterial cells stored at room temperature, and inability to effectively guarantee the number of viable bacteria

Active Publication Date: 2018-04-20
金华银河生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] One of the purposes of this application is to provide a strain of Lactobacillus plantarum, and the second purpose of this application is to provide a method for preparing said Lactobacillus plantarum into freeze-dried powder, so as to solve the problem of lactic acid bacteria freeze-dried powder produced by existing freeze-drying methods. The number of viable bacteria in the dry powder is low and the bacteria are easily inactivated when stored at room temperature, and the number of viable bacteria cannot be effectively guaranteed during the shelf life

Method used

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  • Lactobacillus plantarum as well as lactobacillus plantarum freeze-dried powder and preparation method thereof
  • Lactobacillus plantarum as well as lactobacillus plantarum freeze-dried powder and preparation method thereof
  • Lactobacillus plantarum as well as lactobacillus plantarum freeze-dried powder and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1～4

[0088] The selection of the amount of small molecule sugar in the bacterium protection agent of embodiment 1~4

[0089] Take 1 kg of wet bacteria prepared in (1), add 1 kg of protein (equal weight composition of skim milk powder and desalted whey powder), and add different amounts of small molecular sugars (equal weight of trehalose, lactose, sucrose and fructose) Composition) was freeze-dried under the same conditions for 48 hours to prepare Lactobacillus plantarum LP4 freeze-dried powder, and the prepared Lactobacillus plantarum LP4 freeze-dried powder was stored at 25°C for 6 months, and the survival rate of viable bacteria was determined, as shown in the following table 1 shows:

[0090] Table 1

[0091]

[0092] After comparative analysis, it was found that during the vacuum freeze-drying process of Lactobacillus plantarum LP4, the addition of small molecular sugars (an equal weight composition of trehalose, lactose, sucrose and fructose) can effectively protect the...

Embodiment 5～9

[0094] The influence of embodiment 5~9 polyhydric alcohol on plantaractobacillus LP4 freeze-dried powder

[0095] Get 1 kg of wet thalline prepared in (1), add protein (equal weight composition of skim milk powder and desalted whey powder) 1 kg, small molecular sugar (equal weight composition of trehalose, lactose, sucrose and fructose) ) 1000g, on its basis, add polyhydric alcohols in different proportions (the weight of glycerol: the weight of mannitol: the composition of the weight of sorbitol=2:1:1), vacuum freeze-drying under the same conditions for 48 hours was prepared into plant Lactobacillus LP4 freeze-dried powder was stored at 25°C for 6 months, and the survival rate of live bacteria was determined, as shown in Table 2 below:

[0096] Table 2

[0097]

[0098] Through comparative analysis, it is found that in the vacuum freeze-drying process, adding polyhydric alcohol (weight of glycerol: weight of mannitol: weight of sorbitol=2:1:1 composition) can effectivel...

Embodiment 10～13

[0100] The influence of embodiment 10~13 polysaccharide dosage on Lactobacillus plantarum LP4 freeze-dried powder

[0101] Get 1 kg of wet thalline prepared in (1), add protein (skimmed milk powder: equal weight composition of desalted whey powder) 1 kg, small molecule sugar (equal weight composition of trehalose, lactose, sucrose and fructose) ) 1000g, polyol (the weight of glycerin: the weight of mannitol: the composition of the weight of sorbitol=2:1:1) 400g, on its basis, add polysaccharides in different proportions, specifically, after adding polyol for the first time Add chitosan, then add non-alkaline polysaccharide composition (equal weight composition of gelatin, maltodextrin, sodium alginate, gum arabic), then add chitosan twice, vacuum lyophilize 48h under the same conditions, The freeze-dried powder of Lactobacillus plantarum LP4 was prepared, stored at 25°C for 6 months, and the survival rate of live bacteria was determined, as shown in Table 3 below:

[0102] ...

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Abstract

The application provides lactobacillus plantarum LP4; the microbial preservation number of the lactobacillus plantarum LP4 is CGMCC No. 14533. The application also provides a method for preparing lactobacillus plantarum LP4 freeze-dried powder, and the lactobacillus plantarum LP4 freeze-dried powder prepared by using the method; according to the method, the lactobacillus plantarum LP4 is coated before being freeze-dried, and multiple protective agents are used in a combined way; in particular, after the surface of the lactobacillus plantarum LP4 is coated for the first time, secondary coatingis performed, so that the defect of incomplete coating which is possibly caused during the first coating can be overcome; a completely dense protective layer is at least formed on the surface of the lactobacillus plantarum LP4 and forms a microcapsule structure together with a thallus, so that the lactobacillus plantarum LP4 thallus is effectively isolated from the external environment, and a stable internal environment is formed inside the microcapsule; therefore, the lactobacillus plantarum LP4 is enabled to maintain viability under the room temperature condition.

Description

technical field [0001] The application relates to the field of biotechnology, in particular to a strain of Lactobacillus plantarum, the freeze-dried powder of Lactobacillus plantarum and a preparation method thereof. Background technique [0002] Compared with lactic acid bacteria cryogenic preparations, lactic acid bacteria freeze-dried powder is widely used due to its advantages of easy storage and use. Stability indicators such as the number of viable bacteria, survival rate, and vigor of the bacteria in the lactic acid bacteria freeze-dried powder have an important impact on whether it can be used normally. [0003] Usually, the method of freeze-drying lactic acid bacteria is to rapidly freeze the lactic acid bacteria cells containing the liquid phase components in a low temperature environment, and then obtain the lactic acid bacteria freeze-dried powder according to the temperature program. Shock can lead to changes in cell wall permeability and metabolic damage, resu...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/04C12R1/25
CPCC12N1/04C12N1/20C12N1/205C12R2001/25
Inventor 姚国强张建军
Owner 金华银河生物科技有限公司
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