Lactobacillus plantarum as well as lactobacillus plantarum freeze-dried powder and preparation method thereof

A technology of Lactobacillus plantarum and freeze-dried powder, applied in the biological field, can solve the problems of low number of viable bacteria, easy inactivation of bacterial cells stored at room temperature, and inability to effectively guarantee the number of viable bacteria
CN107937320AActive Publication Date: 2018-04-20金华银河生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
金华银河生物科技有限公司
Publication Date
2018-04-20

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Abstract

The application provides lactobacillus plantarum LP4; the microbial preservation number of the lactobacillus plantarum LP4 is CGMCC No. 14533. The application also provides a method for preparing lactobacillus plantarum LP4 freeze-dried powder, and the lactobacillus plantarum LP4 freeze-dried powder prepared by using the method; according to the method, the lactobacillus plantarum LP4 is coated before being freeze-dried, and multiple protective agents are used in a combined way; in particular, after the surface of the lactobacillus plantarum LP4 is coated for the first time, secondary coatingis performed, so that the defect of incomplete coating which is possibly caused during the first coating can be overcome; a completely dense protective layer is at least formed on the surface of the lactobacillus plantarum LP4 and forms a microcapsule structure together with a thallus, so that the lactobacillus plantarum LP4 thallus is effectively isolated from the external environment, and a stable internal environment is formed inside the microcapsule; therefore, the lactobacillus plantarum LP4 is enabled to maintain viability under the room temperature condition.
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Description

technical field

[0001] The application relates to the field of biotechnology, in particular to a strain of Lactobacillus plantarum, the freeze-dried powder of Lactobacillus plantarum and a preparation method thereof. Background technique

[0002] Compared with lactic acid bacteria cryogenic preparations, lactic acid bacteria freeze-dried powder is widely used due to its advantages of easy storage and use. Stability indicators such as the number of viable bacteria, survival rate, and vigor of the bacteria in the lactic acid bacteria freeze-dried powder have an important impact on whether it can be used normally.

[0003] Usually, the method of freeze-drying lactic acid bacteria is to rapidly freeze the lactic acid bacteria cells containing the liquid phase components in a low temperature environment, and then obtain the lactic acid bacteria freeze-dried powder according to the temperature program. Shock can lead to changes in cell wall permeability and metabolic damage, resu...

Claims

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