Epinephelus coioides insulin gene, coded protein and application thereof

A pet22b-insulin and oblique grouper technology, applied in the field of genetic engineering, can solve the problems of lower production cost and high cost of animal and plant proteins, and achieve the effect of reducing usage and production cost
CN107937408AActive Publication Date: 2018-04-20SUN YAT SEN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SUN YAT SEN UNIV
Publication Date
2018-04-20

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Abstract

The invention belongs to the technical field of genetic engineering, and in particular discloses an epinephelus coioides insulin gene, coded protein and an application thereof. The nucleotide sequenceof the insulin protein is shown as SEQ ID NO:1, and the amino acid sequence of the insulin protein coded by the gene is shown as SEQ ID NO.2. The epinephelus coioides insulin gene provided by the invention is obtained for the first time through cloning, and the gene is applied to construction of an expression vector and a recombinant escherichia coli strain; the recombinant epinephelus coioides insulin protein, which is high in expression, is obtained on the basis of the recombinant escherichia coli strain in vitro; and the protein, which can promote glycolipid utilization and growth of suchmarine fishes as epinephelus coioides and the like, can be used for preparing growth promoters or bait additives applicable to the marine fishes; therefore, the protein has a relatively broad application prospect.
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Description

technical field

[0001] The invention relates to the technical field of genetic engineering, in particular to a kind of insulin Genes, encoded proteins and their applications. Background technique

[0002] Insulin plays an important regulatory role in the metabolic regulation of the body. The pancreas has always been considered as an important insulin-producing organ, but recent studies have shown that other organs besides the pancreas can also secrete insulin. As an important hormone, insulin plays an important role in the growth, cell differentiation and metabolism of the body. As one of the earliest discovered insulins, fish insulin plays an important role in many aspects such as fish feeding, growth, development and regulation of glucose and lipid metabolism. After insulin binds to the receptor, it can trigger a variety of downstream biological functions through PI3K / Akt, ERK and STAT / Jak–STAT signaling pathways.

[0003] Grouper (grouper) Serranidae ), Grouper ( E...

Claims

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