Mitochondria transferring solution and preparation method thereof

A mitochondria and buffer technology, applied in the field of cell or organelle treatment solution, can solve the problems of reducing the overall transfer efficiency of mitochondria, damage mitochondrial activity, complex serum components, etc., to reduce research and development costs, increase transfer, and increase endocytosis. Effect

Inactive Publication Date: 2018-05-08
佳学基因医学技术(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the cell culture medium, there are generally abundant calcium ions and animal serum; the presence of calcium ions will damage the activity of mitochondria, thereby reducing the overall transfer efficiency of mitochondria; and because the composition of serum is too complex, if use

Method used

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  • Mitochondria transferring solution and preparation method thereof
  • Mitochondria transferring solution and preparation method thereof
  • Mitochondria transferring solution and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A mitochondrial transfer fluid, the combination and dosage of the mitochondrial transfer fluid are shown in Table 1:

[0042] Table 1: Composition of Mitochondrial Transfer Solution

[0043]

[0044] Total volume: 1L; among them, JXNANO-coupling agent is RETWWETWWTEWSQPRRRRKV-mercaptoethylamine.

[0045] The preparation method of the mitochondrial transfer fluid is:

[0046] Take a 1L measuring cup, add 600ml of ultrapure water, respectively take each component of the mitochondrial transfer solution, add them to the ultrapure water in order, and add while stirring until all are dissolved. Adjust the pH value to 7.0 with 1M hydrochloric acid or NaOH, continue to add ultrapure water to 950ml, adjust the pH value to pH 7.0 with 1M hydrochloric acid or NaOH, add ultrapure water to 1 liter, filter and sterilize with a 0.25um filter membrane , Put it in the refrigerator at 4 degrees.

Embodiment 2

[0048] A mitochondrial transfer fluid, the combination and dosage of the mitochondrial transfer fluid are shown in Table 2:

[0049] Table 2: Composition of Mitochondrial Transfer Solution

[0050]

[0051] Total volume: 1L; among them, JXNANO-coupling agent is RETWWETWWTEWSQPRRRRKV-mercaptoethylamine.

[0052] The preparation method of the mitochondrial transfer fluid is:

[0053] Take a 1L measuring cup, add 600ml of ultrapure water, respectively take each component of the mitochondrial transfer solution, add them to the ultrapure water in order, and add while stirring until all are dissolved. Adjust the PH value to 7.4 with 1M hydrochloric acid or NaOH, continue to add ultrapure water to 950ml, adjust the pH to pH 7.4 with 1M hydrochloric acid or NaOH, add ultrapure water to 1 liter, filter and sterilize with a 0.25um filter membrane , Put it in the refrigerator at 4 degrees.

Embodiment 3

[0055] A mitochondrial transfer fluid, the combination and dosage of the mitochondrial transfer fluid are shown in Table 3:

[0056] Table 3: Composition of Mitochondrial Transfer Solution

[0057]

[0058] Total volume: 1L; among them, JXNANO-coupling agent is RETWWETWWTEWSQPRRRRKV-mercaptoethylamine.

[0059] The preparation method of the mitochondrial transfer fluid is:

[0060] Take a 1L measuring cup, add 600ml of ultrapure water, respectively take each component of the mitochondrial transfer solution, add them to the ultrapure water in order, and add while stirring until all are dissolved. Adjust the pH to 7.2 with 1M hydrochloric acid or NaOH, continue to add ultrapure water to 950ml, adjust the pH to pH 7.2 with 1M hydrochloric acid or NaOH, add ultrapure water to 1 liter, filter and sterilize with a 0.25um filter membrane , Put it in the refrigerator at 4 degrees.

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Abstract

The invention provides a mitochondria transferring solution and a preparation method thereof. The mitochondria transferring solution does not contain calcium ions and animal serum; the mitochondria transferring solution can keep the activity of mitochondria, and does not cause rejection and anaphylaxis of a living body; the requirements of mitochondria transferring also can be met and the mitochondria transferring solution can be used in vitro and in vivo. The preparation method of the mitochondria transferring solution is simple to operate and is suitable for large-scale popularization and application.

Description

Technical field [0001] The invention belongs to the technical field of cell or organelle treatment liquid, and specifically relates to a high-efficiency mitochondrial transfer liquid and a preparation method thereof. Background technique [0002] Mitochondria are organelles that provide energy to cells. It has its own set of DNA (mtDNA), which is passed on to the next generation through the mother's eggs. Mitochondrial mutations that occur in eggs may cause maternal familial diseases. This mutation can cause serious problems. Most of the affected organs are high-energy-demanding organs, such as the brain, muscles, and heart, and involve a wide range of maternal genetic diseases. The DNA sequence in the mitochondria can also produce mutations during development and aging. This new mutation only appears in the patient, not in the biological mother. The current treatment options are extremely limited, and patients can only rely on drugs for short-term relief of symptoms and cannot...

Claims

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Application Information

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IPC IPC(8): C12N5/071
CPCC12N5/0602C12N2500/30C12N2500/32C12N2500/40C12N2501/999
Inventor 黄家学杨萍
Owner 佳学基因医学技术(北京)有限公司
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