A liquid chromatogram-tandem-mass spectrometry method for simultaneously detecting five types of medicines in animal edible products and feed

A technology of liquid chromatography and tandem mass spectrometry is applied in the field of detection of various drug residues to achieve the effects of low reagent cost, shortened processing time and simple operation process.

Inactive Publication Date: 2018-05-15
HUAZHONG AGRI UNIV
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Problems solved by technology

[0005] The currently reported research on the detection of prohibited feed additives needs to be improved in terms of drug detection and matrix monitoring.
Screening methods for the simultaneous detection of adrenergic drugs, steroid hormones, psychotropic drugs, antioxidants and pigments in multiple matrices have not yet been developed

Method used

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  • A liquid chromatogram-tandem-mass spectrometry method for simultaneously detecting five types of medicines in animal edible products and feed
  • A liquid chromatogram-tandem-mass spectrometry method for simultaneously detecting five types of medicines in animal edible products and feed
  • A liquid chromatogram-tandem-mass spectrometry method for simultaneously detecting five types of medicines in animal edible products and feed

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Experimental program
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Embodiment Construction

[0028] The present invention will be described in detail below by way of examples.

[0029] (1) Sample pretreatment:

[0030] Extraction Accurately weigh 2.0g of the sample and place it in a 50mL centrifuge tube, add 5mL of acetonitrile-ethyl acetate (1:1), vortex for 2min, centrifuge at 8000r / min for 5min, add 5mL of 1% ammonia-acetonitrile-ethyl acetate to the remaining residue The solution was vortexed for 2 minutes, and centrifuged at 8000 r / min for 5 minutes. And and two extracts, mix. After nitrogen blowing, redissolve with 5mL methanol and wait for purification.

[0031] Purify the solid-phase extraction column with 5mL of methanol and water to activate respectively, keep the column wet, load the sample, discard the effluent, rinse with 3mL of water, elute with 10mL of acetonitrile-ethyl acetate (1:1), and elute The solution was blown dry with nitrogen in a water bath at 30°C, reconstituted with 1 mL of the initial mobile phase, and determined by LC-MS / MS.

[0032] ...

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Abstract

A liquid chromatogram-tandem-mass spectrometry method for simultaneously detecting five types of medicines in animal edible products and feed is disclosed. The five types of medicines are epinephrinetype medicines, steroid hormones, psychotropic medicines, coloring agents and antioxidants. The method belongs to the technical field of medicine residual detection and analysis. The method includes two steps, namely a step of sample pretreatment and a step of liquid chromatogram-tandem-mass spectrometry detection. The method enriches and completes present detection techniques, can simultaneouslymeasure 88 medicines, and is simple, convenient and rapid to operate, and suitable for simultaneous detection of a plurality of medicines in feed and a plurality of animal foods.

Description

technical field [0001] The invention belongs to the technical field of drug residue detection and analysis, and relates to a detection method for various drug residues in animal tissues and feed. Background technique [0002] With the development of society, the aquaculture industry has also undergone tremendous changes, gradually showing the development trend of scale and intensification. How to ensure the safety of feed and animal food has become a major problem in the breeding industry. In the early days, many drugs that can effectively promote animal growth were widely used in feed addition, such as adrenaline drugs, sex hormones, etc. Then a series of food safety problems such as poisoning incidents occurred. With the continuous development of science and technology, the use of drugs in the breeding process is also becoming more and more perfect. In 2010, the Ministry of Agriculture of my country promulgated a catalog of drugs that are prohibited from being used in l...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 陶燕飞朱愈斐谢书宇陈冬梅王旭潘源虎黄玲利王玉莲刘振利彭大鹏戴梦红郝海红程古月瞿伟
Owner HUAZHONG AGRI UNIV
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