Color recognition based quantitative measurement method for concentration of to-be-measured liquid

Abstract

The invention relates to the field of biological detection, in particular to a color recognition based quantitative measurement method for concentration of a to-be-measured liquid. The method comprises the following main steps: S4, locating information corresponding to a color reaction area and each color block with an image recognition technology, and converting diffuse reflection light information into color coordinates in specific color space; S5, equivalently reckoning diffuse reflection information of the color reaction area and the color blocks, environment illumination and other external factors into equivalent environment illumination on the basis of principles of optics; S7, calculating standard color of the color reaction area; S8, calculating the concentration of the to-be-measured liquid according to the corresponding relation between the standard color of the color reaction area and the concentration of the to-be-measured liquid, and outputting a result. Compared with theprior art, the color recognition based quantitative measurement method for the concentration of the to-be-measured liquid has the advantages that measurement errors caused by equipment and environmentfactors can be further corrected, measurement precision is improved, cost is reduced, and applicability is improved.

Description

【Technical field】 [0001] The invention relates to the field of biological detection, in particular to a color recognition-based quantitative detection method for the concentration of a liquid to be tested. 【Background technique】 [0002] All kinds of chemical substances often change color after chemical reaction (including complexation reaction, chelation reaction, redox reaction, etc.), and at this time it can be said that it has undergone a color change reaction. The essence of the discoloration reaction is that the degree of individual or individual aggregation of chemical reagent molecules changes before and after the reaction (molecular particle diameter or intermolecular gap changes), so that it loses or acquires the ability to absorb or scatter visible light of a specific wavelength, which is manifested in the human eye Color change. [0003] The color-changing ability of chemical reagents is often used for the characterization or identification of specific chemical ...

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Problems solved by technology

[0022] However, in the current mainstream method, whether it is the technical solution of using photoelectric sensor to obtain "absorbance" or the technical solution of using color detection to obtain color coordinate values, it is necessary to control the illumination on the surface of the reagent strip, which means that it is likely to use detection The device creates a closed and dark environment, which increases the size and cost of the equipment, and limits the economy and applicability of portable equipment based on this principle

[0023] There has been a patent scheme describing the use of a handheld device with a camera to directly take pictures of the reagent strips, and to judge the concentration of the analyte based on the acquired color of the discoloration area of ​​the reagent strips (CN103649731A-WO2012131386A1), but the default environment for taking pictures is The illumination light is given by a handheld device, and the test environment described in the scheme is completely idealized, which cannot guarantee the effectiveness of the scheme in the real environment

[0024] In the scheme of "contrast color card" introduced in the semi-quantitative test (US9285323B2), it can only avoid the measurement error caused by the "ambient light change" of the specific white light intensity change, but cannot adapt to changes in other lighting conditions.

At the same time, it is difficult to ensure that the color of the "comparison color card" itself is completely consistent with the color of the color-changing area of ​​the reagent strip that actually corresponds to each concentration of the test substance, which will also introduce measurement errors

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