Transgenic method for increasing oil peony seed oil content

Abstract

The invention belongs to the field of bioengineering and provides a transgenic method for increasing oil peony seed oil content. The method includes: constructing a polygene plant expression vector; transferring into a peony plant tissue through agrobacterium tumefaciens to realize expression and heredity, wherein construction of the polygene plant expression vector includes that a polycloning site is introduced at a 3' terminal of a 35S promoter on Pbi1211-GUS to construct a vector Pbi121-35S MCS, and exogenous genes WRI1, LEC2 and FAD2 are sequentially inserted into Pbi121-35S MCS to form the polygene plant expression vector Pbi121-WRI1-LEC2-FAD2. On the basis of the constructed polygene plant expression vector, effective expression in peony plants can be realized, and harvested peony seeds can be stable in inheritance and high in oil content.

Description

technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to a transgenic method for increasing the oil content of peony seeds for oil. Background technique [0002] Peony is a unique traditional famous flower in my country. It has a cultivation history of more than 2,000 years and there are more than 800 cultivars. Peony is of great value in ornamental, medicinal, and health care. High economic value and edible and medicinal value have attracted widespread attention. Generally, the oil content of peony seeds for oil is between 27% and 33%. In practice, traditional breeding or changing cultivation conditions are usually used to increase the oil content of peony seeds, but these The randomness of the method is strong, and the increase rate is small. It is very difficult to increase the oil content by relying on traditional breeding methods only through phenotypic selection. Especially in the face of the current rapid development of ...

AI Technical Summary

Problems solved by technology

[0002] Peony is a unique traditional famous flower in my country. It has a cultivation history of more than 2,000 years and there are more than 800 cultivars. Peony is of great value in ornamental, medicinal, and health care. High economic value and edible and medicinal value have attracted widespread attention. Generally, the oil content of peony seeds for oil is between 27% and 33%. In practice, traditional breeding or changing cultivation conditions are usually used to increase the oil content of peony seeds, but these The randomness of the method is strong, and the increase rate is small. It is very difficult to increase the oil content by relying on traditional breeding methods only through phenotypic selection.

Especially in the face of the current rapid development of the peony industry, relying on traditional breeding techniques is far from meeting the needs of large-scale planting and production, and it is even more difficult to meet the needs of industrialized deep processing

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