Tissue culture method for rapid propagation of bletilla striata
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A Bletilla striata and fast technology is applied in the field of tissue culture for rapid propagation of Bletilla striata and can solve the problems of long period of test-tube seedling formation and high cost, and achieve the effects of improving germination time and promoting population protection.
Inactive Publication Date: 2018-06-01
临沧市云瑞堂生物科技有限公司
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Problems solved by technology
[0003] At present, there have been relevant reports on the tissue culture research of Bletilla striata, but the high cost of tissue culture and the long cycle of test tube seedlings are the bottlenecks restricting the large-scale industrial production of Bletilla striata
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Embodiment 1
[0028] A method for tissue culture of rapid propagation of bletilla striata into seedlings, comprising the following steps:
[0029] 1) Aseptic germination of Bletilla striata seeds
[0030] Bletilla striata capsule seeds were collected, sterilized with alcohol and sodium hypochlorite solution sequentially, one end of the sterilized seeds was cut open, the powdery embryos were evenly spread on the seeding medium, and cultivated in a lighted tissue culture room for 14 days, The germinated seeds form protocorms, and the temperature of the tissue culture room is kept at 22°C;
[0031] 2) Proliferation of Bletilla striata protocorm
[0032] Evenly transfer the protocorm formed by seed germination into the proliferation medium, place it in the tissue culture room for light culture, keep the temperature of the tissue culture room at 22°C, the protocorm grows a light yellow callus, and after 22 days, the callus Continuously separate and proliferate into protocorms;
[0033] 3) Dif...
Embodiment 2
[0046] A method for tissue culture of rapid propagation of bletilla striata into seedlings, comprising the following steps:
[0047] 1) Aseptic germination of Bletilla striata seeds
[0048] Collect the Bletilla striata capsule seeds, disinfect and sterilize them with alcohol and sodium hypochlorite solution in sequence, cut off one end of the sterilized seeds, spread the powdery embryos evenly on the seeding medium, and cultivate them in a lighted tissue culture room for 25 days. The germinated seeds form protocorms, and the temperature of the tissue culture room is kept at 25°C;
[0049] 2) Proliferation of Bletilla striata protocorm
[0050] Evenly transfer the protocorm formed by seed germination into the proliferation medium, place it in the tissue culture room for light culture, keep the temperature of the tissue culture room at 25°C, and the protocorm grows a light yellow callus. After 27 days, the callus Continuously separate and proliferate into protocorms;
[0051...
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Abstract
The invention discloses a tissue culture method for rapid propagation of bletilla striata. The method comprises the following steps: 1) aseptic germination of seeds of the bletilla striata; 2) proliferation of protocorm of the bletilla striata; 3) differentiation of protocorms of the bletilla striata; 4) strengthening seedlings of small seedlings of the bletilla striata; 5) rooting of big seedlings of the bletilla striata. The tissue culture method has the advantages that: the invention provides the tissue culture method for the rapid propagation of the bletilla striata to cultivate seedlings,a series of rapid propagation mediums of the bletilla striata can significantly improve the germination time of the bletilla striata seeds, and the germination time is directly shortened from the traditional time of about 100 days to 14 to 25 days, the germination rate of wild seeds is as high as 93 to 96.2%, and the survival rate of tissue culture seedlings of the bletilla striata is as high as98%. The components of a proliferation medium, a seedling strengthening medium, a differentiation medium, and a rooting medium are adjusted, so that the growing bletilla striata seedlings are robust and the transplanting survival rate is as high as 98%. The tissue culture method plays a very good role in promoting the population protection of the bletilla striata of medicinal plants of endangeredOrchidaceae, and lays the foundation of biotechnology for the realization of the value of valuable traditional Chinese medicinal materials.
Description
technical field [0001] The invention belongs to the field of planting medicinal plants, and in particular relates to a tissue culture method for rapid propagation and seedling formation of bletilla striata. Background technique [0002] Bletilla striata belongs to Bletilla striata, also known as Lianjicao, Gangen, Baiji, Ruolan, Zhulan, Zilan, Zihui, Baili. Perennial herbaceous bulbous plants (root tubers), 18-60 cm high. Mainly distributed in China, Japan and northern Myanmar. The main flowering period is in spring, but depending on the climate of various places, it may bloom from late winter to early summer. Bletilla striata has a wide range of medicinal value and garden value, mainly used for astringent hemostasis, detumescence and muscle growth. The flowers have purple, white, blue, yellow and pink colors, and can be potted indoors for viewing, or decorated in a relatively shaded flower bed, flower border or corner of a courtyard. The pharmacological effects of Bleti...
Claims
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