Enrichment method of tobacco glucoside
A technology for tobacco and glycosides, applied in the directions of tobacco, tobacco processing, application, etc., can solve the problems of high preparation cost, unsuitable for large-scale production, difficult to obtain glycoside mixture, etc., and achieve cost advantages and environmental friendly effects.
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Embodiment 1
[0039] A method for enriching tobacco glycosides, comprising the steps of:
[0040] Step (1), preparation of the tobacco suspension: after centrifuging the water extract of the tobacco raw material with a dry matter content of 15%, discarding the precipitate to obtain the tobacco suspension;
[0041] The tobacco raw material is tobacco stem;
[0042] Described chemical sedimentation is that 0.2% chitosan is sedimentation agent with mass concentration;
[0043] Step (2), one-time filtration: filter the tobacco suspension liquid obtained in step (1) through a 100nm pore size membrane, and when the volume ratio of the retentate and permeate is 1:19, add a volume of tobacco suspension liquid to the retentate Liquid volume 1 / 10 of the water, after mixing, continue to pass through the 100nm pore size membrane until the volume of the final retained liquid is 5% of the volume of the tobacco suspended turbid liquid, and the filtration is completed;
[0044] Step (3), secondary filtra...
Embodiment 2
[0051] A method for enriching tobacco glycosides, comprising the steps of:
[0052] Step (1), preparation of the tobacco suspension: after centrifuging the water extract of the tobacco raw material with a dry matter content of 6%, discarding the precipitate to obtain the tobacco suspension;
[0053] The tobacco raw material is tobacco powder;
[0054] Described chemical sedimentation is that 0.2% chitosan is sedimentation agent with mass concentration;
[0055] Step (2), one-time filtration: filter the tobacco suspension liquid obtained in step (1) through a 100nm pore size membrane, and when the volume ratio of the retentate and permeate is 1:19, add a volume of tobacco suspension liquid to the retentate Liquid volume 1 / 10 of the water, after mixing, continue to pass through the 100nm pore size membrane until the volume of the final retained liquid is 5% of the volume of the tobacco suspended turbid liquid, and the filtration is completed;
[0056] Step (3), secondary filtr...
Embodiment 3
[0063] A method for enriching tobacco glycosides, comprising the steps of:
[0064] Step (1), preparation of the tobacco suspension: after chemical precipitation of the water extract of the tobacco raw material with a dry matter content of 25%, discarding the precipitate to obtain the tobacco suspension;
[0065] The tobacco raw material is tobacco leaves;
[0066] Described chemical sedimentation uses diatomaceous earth as sedimentation agent;
[0067] Step (2), one-time filtration: filter the tobacco suspension liquid obtained in step (1) through a 100nm pore size membrane, and when the volume ratio of the retentate and permeate is 1:19, add a volume of tobacco suspension liquid to the retentate Liquid volume 1 / 10 of the water, after mixing, continue to pass through the 100nm pore size membrane until the volume of the final retained liquid is 5% of the volume of the tobacco suspended turbid liquid, and the filtration is completed;
[0068] Step (3), secondary filtration: f...
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