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Culture dish device and cell membrane sheet culture method

A cell membrane and petri dish technology, which is applied in general culture methods, tissue cell/virus culture devices, and biological material sampling methods, etc., can solve problems such as the adverse effects of cell membrane growth, the heat source, and the large amount of heat emitted by the light source. , to achieve the effect of facilitating effective growth, realizing real-time detection, and reducing adverse effects

Active Publication Date: 2018-06-01
BOE TECH GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the commonly used cell membrane preparation device is a temperature-sensitive culture dish device. However, in the existing temperature-sensitive culture dish device, the thermal light source and the heat generated by the light source are relatively large, which is likely to have an adverse effect on the growth of the cell membrane.

Method used

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  • Culture dish device and cell membrane sheet culture method
  • Culture dish device and cell membrane sheet culture method
  • Culture dish device and cell membrane sheet culture method

Examples

Experimental program
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Effect test

Embodiment 1

[0047] Such as figure 1 As shown, the present embodiment provides a petri dish device, comprising a dish body (101), the interior of the dish body (101) is provided with a temperature sensitive layer (106) and a luminescence structure (108), and the luminescence structure (108 ) is used to emit luminescent light, and a separation layer (107) is arranged between the temperature sensitive layer (106) and the luminescent structure (108).

[0048] Such as figure 1 As shown, the cell membrane (105) to be cultured is placed on the temperature-sensitive layer (106), and the temperature-sensitive layer (106) is used as the base layer for the generation of the cell membrane, so as to facilitate the later harvest and collection of the cell membrane. Compared with common thermal light sources, the cold light structure (108) of this embodiment emits cold light to the cell membrane, which emits less heat and can reduce the adverse effect of the light source on the growth of the cell membr...

Embodiment 2

[0050] This embodiment provides a petri dish device, comprising a dish body (101), the interior of the dish body (101) is provided with a temperature sensitive layer (106) and a luminescence structure (108), and the luminescence structure (108) is used for Luminescent light is emitted, and a separation layer (107) is arranged between the temperature sensitive layer (106) and the luminescent structure (108). The luminescent structure (108) includes scattering particles, and the scattering particles are uniformly dispersed in the luminescent structure (108), and the luminescent light source used for emitting from the luminescent structure (108) is evenly scattered out.

[0051] Optionally, the scattering density of the scattering particles around the luminescent structure (108) is greater than that at the center, which is beneficial to better scatter the luminescent LED light source around the luminescent structure to irradiate the cell growth layer.

[0052] Optionally, the sca...

Embodiment 3

[0054] This embodiment provides a petri dish device, comprising a dish body (101), the interior of the dish body (101) is provided with a temperature sensitive layer (106) and a luminescence structure (108), and the luminescence structure (108) is used for Luminescent light is emitted, and a separation layer (107) is arranged between the temperature sensitive layer (106) and the luminescent structure (108). Such as figure 2 As shown, the cold light structure (108) includes a plurality of cold light LED light sources (110), and the cold light LED light sources (110) are arranged in an array in the cold light structure (108), utilizing less heat than ordinary light sources The cold light LED light source uniformly emits the cold light light source to the cell membrane, which can reduce the adverse effect of the light source on the growth of the cell membrane.

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Abstract

The invention provides a cell membrane sheet culture dish device and a cell membrane sheet culture method. A temperature sensitive layer, a cold light structure and a temperature regulating layer arearranged in the culture vessel device; the temperature environment in a culture dish is regulated and controlled through the temperature sensitive layer and the temperature regulating layer; a cell membrane sheet is enabled to grow under the proper temperature conditions; the cold light structure with less heat emission compared to an ordinary light source is used for uniformly emitting cold lightto a direction of the cell membrane sheet; the adverse influence of heat emission of the light on the growth of the cell membrane sheet can be effectively reduced; the real-time detection on the growth parameter of the cell membrane sheet can be realized.

Description

technical field [0001] The invention relates to a device structure design of a culture dish and a method for cultivating cell membranes. Background technique [0002] Cell sheet is a research hotspot in the field of tissue engineering in recent years, and has a wide range of applications in the treatment of skin, cornea, heart, periodontal and other related diseases. The currently commonly used device for preparing cell membranes is a temperature-sensitive culture dish device. However, in the existing temperature-sensitive culture dish devices, the thermal light source and the heat generated by the light source are relatively large, which is likely to have an adverse effect on the growth of the cell membrane. Contents of the invention [0003] (1) Technical problems to be solved [0004] In view of the above technical problems, embodiments of the present invention provide a culture dish device capable of effectively controlling the temperature of the cell membrane culture...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/04C12M1/22
CPCC12M23/10C12M23/20C12M23/46C12M31/10C12M41/12C12M25/02C12M33/00C12N5/0068
Inventor 周适
Owner BOE TECH GRP CO LTD
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