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Rh blood type typing method and device based on multiplex PCR (Polymerase Chain Reaction) and second-generation sequencing

A technology of next-generation sequencing and rh blood type, applied in the field of bioinformatics, can solve the problems of inaccurate test results and easy occurrence of false positives, and achieve the effect of avoiding false positives, avoiding misjudgment of typing results, and improving accuracy.

Active Publication Date: 2018-06-01
CAPITALBIO GENOMICS
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Problems solved by technology

Due to the extremely high similarity between RHD and RHCE genes, there are often misjudgments in the RH blood group analysis based on multiplex PCR and next-generation sequencing, resulting in inaccurate test results, especially prone to false positives

Method used

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  • Rh blood type typing method and device based on multiplex PCR (Polymerase Chain Reaction) and second-generation sequencing
  • Rh blood type typing method and device based on multiplex PCR (Polymerase Chain Reaction) and second-generation sequencing
  • Rh blood type typing method and device based on multiplex PCR (Polymerase Chain Reaction) and second-generation sequencing

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Embodiment Construction

[0031] Due to the extremely high similarity between RHD and RHCE genes, there are often misjudgments in Rh blood typing analysis based on multiple PCR gene capture and next-generation sequencing, resulting in low accuracy and false positives. In order to solve this problem , the inventor discovered the cause of the wrong comparison, and designed an error correction scheme in a targeted manner, and developed a Rh blood type typing method and device with high accuracy.

[0032] refer to figure 1 , the Rh blood typing method based on multiplex PCR and next-generation sequencing, comprising the following steps:

[0033] S1: Using multiplex PCR primers designed for the whole exons of the RHD gene, construct the amplicon library of the sample to be tested, and use the next-generation sequencing platform to sequence the amplicon library to obtain the sequencing fragment sequence.

[0034] Among them, due to the high similarity between the RHD and RHCE genes, the multiplex PCR primer...

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Abstract

The invention discloses an Rh blood type typing method and device based on multiplex PCR (Polymerase Chain Reaction) and second-generation sequencing. RHD (Rheumatic Heart Disease) and RHCE genes havevery high similarity; during the amplification comparison to the human genome reference sequence, the problem of wrong comparison easily occurs. A multiplex PCR primer sequence is cut and removed before the comparison; the matching precision of the sequence can be improved; the wrong judgment on the typing result caused by wrong comparison is avoided; the Rh blood type typing accuracy rate is greatly improved; the occurrence of false positive result can be avoided.

Description

technical field [0001] The invention belongs to the field of bioinformatics, and in particular relates to a Rh blood typing method and device based on multiplex PCR and next-generation sequencing. Background technique [0002] At present, among the 29 red blood cell blood group systems confirmed by the International Society of Blood Transfusion (ISBT), the blood group system with the most polymorphism and complexity is the Rh blood group system. It is traditionally believed that the Rh blood group system is second only to the ABO blood group system in clinical importance. Rh blood group is related to hemolytic transfusion reaction, hemolytic disease of the newborn, and autoimmune hemolytic anemia. necessary testing items. [0003] Rh blood type is the most important blood group phenotype in the Rh blood group system. According to whether the red blood cell surface contains D antigen, the Rh blood type is divided into Rh positive and Rh negative. Among them, Rh positive has ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/686C12Q1/6869G01N33/80
CPCC12Q1/686C12Q1/6869C12Q1/6888G01N33/80C12Q2537/143C12Q2535/122
Inventor 糜庆丰朱鹏远吴春求潘兆东黄铨飞周幸芝王杨刘丽菲
Owner CAPITALBIO GENOMICS
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