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Application of tfeb as a stroke biomarker and therapeutic target

A stroke and drug technology, applied in the field of biomarkers and therapeutic targets, can solve problems such as unclear effects

Active Publication Date: 2021-02-23
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the role of TFEB in stroke is still unclear. Therefore, it is of great significance to clarify the change rule of TFEB in stroke and find a drug that selectively regulates TFEB for the treatment of stroke.

Method used

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  • Application of tfeb as a stroke biomarker and therapeutic target
  • Application of tfeb as a stroke biomarker and therapeutic target
  • Application of tfeb as a stroke biomarker and therapeutic target

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1: The expression of TFEB in the nucleus at different time points after permanent middle cerebral artery occlusion (pMCAO) in rats and the localization of TFEB in the nucleus at different time points after oxygen-glucose deprivation (OGD) in primary neurons

[0061] 1. Materials:

[0062] experimental animals

[0063] SD rats, male, weighing 260-280g, provided by the Animal Center of Shenyang Pharmaceutical University, animal certificate number: 211002300015664

[0064] cell

[0065] Rat primary cortical neurons: cortical neurons of neonatal rats (less than 24 hours after birth)

[0066] 2. Method:

[0067] 2-1. Establishment of permanent middle cerebral artery occlusion (pMCAO) model in rats

[0068] Preparation of pMCAO model according to Longa method

[0069] (1) Before the pMCAO operation, the rats were fasted for 12 hours, then anesthetized with 3.5% chloral hydrate (0.1mL / kg) intraperitoneally, fixed in a supine position, and cut an incision of abou...

Embodiment 2

[0134] Example 2 : Effect of specific overexpression or knockdown of TFEB on ALP function and ischemic injury after pMCAO

[0135] 1. Materials:

[0136] experimental animals

[0137] SD rats, male, weighing 260-280g, provided by the Animal Center of Shenyang Pharmaceutical University, animal certificate number: 211002300015664

[0138] 2. Method:

[0139] Experimental Design and Grouping

[0140] The first part: 3 hours to 48 hours after pMCAO, the change rule of TFEB and cell location SD male rats, body weight 260-280g, were randomly divided into sham operation group, model group (3, 6, 12, 24, 48 hours), 10 animals per group. The pMCAO model was established, and the cortical brain tissue of 5 animals was isolated at different time points for Western blot detection, and 5 animals were subjected to immunofluorescence detection at the same time to investigate the change rule and cell location of TFEB after pMCAO.

[0141] Part II: Effects of neuron-specific knockdown / ov...

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Abstract

The present invention relates to the application of transcription factor-EB (Transcription factor-EB, TFEB) as a stroke biomarker and therapeutic target. The present invention finds that detecting the expression level of TFEB can be used for the diagnosis of cerebral apoplexy, and the present invention also finds that the overexpression of TFEB can be used for the treatment of cerebral apoplexy. The above findings are of great significance in the diagnosis and treatment of stroke.

Description

technical field [0001] The invention relates to a biomarker and a therapeutic target, in particular to the application of TFEB as a stroke biomarker and a therapeutic target. Background technique [0002] Stroke is a neurological disease caused by various etiologies that cause lesions in the blood vessels supplying the brain. Worldwide, stroke is the leading cause of permanent disability and death in adults. It has become one of the three deadly diseases in the world. The main clinical treatment is thrombolysis, saving dying neurons in the ischemic area (penumbra) and promoting the recovery of nerve function after injury. Prevention and treatment of ischemic cerebrovascular disease is an urgent medical problem for human beings. At present, the US FDA has only approved tissue plasminogen activator (tPA) for thrombolytic therapy after stroke, but its treatment time window is very narrow, and it is only effective if it is used within 4.5 hours of stroke; there are also bleed...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883A61K45/00A61K38/17A61K38/49A61K31/4152A61P9/10
CPCA61K31/4152A61K38/1709A61K38/49A61K45/00C12Q1/6883C12Q2600/158C12Y304/21068A61K2300/00
Inventor 杨静玉吴春福刘月阳
Owner SHENYANG PHARMA UNIVERSITY
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