Ankylosing spondylitis intestinal microbial marker and application thereof

A technology for ankylosing spondylitis and intestinal microorganisms, which is applied in the field of biomedical materials and can solve problems such as intestinal flora imbalance

Pending Publication Date: 2018-06-12
古洁若
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the development of microbial high-throughput sequencing and analysis technology, it has been shown that AS patients have intestinal flora dysbiosis compared with healthy controls

Method used

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  • Ankylosing spondylitis intestinal microbial marker and application thereof
  • Ankylosing spondylitis intestinal microbial marker and application thereof

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Experimental program
Comparison scheme
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Embodiment Construction

[0029] 1. Acquisition of sequencing data

[0030] 1.1 Sample collection and DNA extraction

[0031] The equipment required for sample collection includes: toilet, stool sample collector

[0032] The patients with ankylosing spondylitis were all from the Third Affiliated Hospital of Sun Yat-sen University. The sterile stool sample collector was used to collect fresh stool samples from 41 Chinese Han patients with ankylosing spondylitis. The laboratory divided into EP tubes, each 200mg / part, a total of 5 parts, and immediately stored in a -80°C refrigerator.

[0033] Microbial DNA was extracted using a fecal microbial DNA extraction kit sold in the market according to the instructions, and stored at -80°C.

[0034] 1.2 Construction of library and sequencing

[0035] The DNA library was constructed according to the instrument manufacturer's (Illuminate) 16S rRNA gene amplicon sequencing operation guide. Use agarose gel electrophoresis to detect the purity and concentration of...

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Abstract

The invention relates to ankylosing spondylitis intestinal microbial markers, in particular to an ankylosing spondylitis intestinal microbial marker and application thereof. Detecting abundance variation of the microbial marker can be used for determining probability of individuals with ankylosing spondylitis. The microbial marker and a method for early non-invasive diagnosis of ankylosing spondylitis disease states are provided and can be applied to preparation of medicines for ankylosing spondylitis treatment and balancing of intestinal flora micro-environmental steady states according to different intestinal florae of subjects.

Description

technical field [0001] The present invention relates to ankylosing spondylitis intestinal microbial markers, more specifically: the present invention relates to ankylosing spondylitis markers, a method and device for classifying multiple individuals using ankylosing spondylitis markers, It belongs to the technical field of biomedical materials. Background technique [0002] Ankylosing spondylitis (AS) is a chronic inflammatory disease that mainly invades the spine, involves the sacroiliac joints and surrounding joints, and may be accompanied by extra-articular symptoms such as uveitis, inflammatory bowel disease, and psoriasis. Most of the onset is young adults, the prevalence rate is 0.2-0.5%, and it is more common in men. So far, the etiology of ankylosing spondylitis has not been clarified. Studies suggest that its pathogenesis is mainly determined by genetic susceptibility factors. Environmental factors such as smoking and infection also play an important role in the oc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883
CPCC12Q1/6883C12Q2600/112
Inventor 古洁若
Owner 古洁若
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