Method for rooting and seedling transplantation exercising of vaccinium dunalianum Wight

Abstract

The invention belongs to the technical field of cultivation of vaccinium dunalianum Wight, and discloses a method for rooting and exercising seedling transplanting of vaccinium dunalianum Wight. Basedon proliferation seedlings, analysis is performed in three aspects which are comparison of types and quantity of rooting hormones, screening of a rooting culture medium and screening of an exercisingseedling substrate, the selected basic rooting culture medium is 2.0 mg.L<-1> IBA + 1 / 4 MS + 0.1g.L<-1> activated carbon + 15 g.L<-1> white granulated sugar + 5 g.L<-1> agar. Based on the proliferation seedlings, further analysis is performed in the three aspects which are comparison of the types and quantity of the rooting hormones, screening of the basic rooting culture medium and screening ofthe exercising seedling substrate, so that the rooting rate of the proliferation seedlings and the survival rate of exercising seedlings of vaccinium dunalianum Wight are improved, and a certain scientific basis and certain technical support are provided for factory cultivation of vaccinium dunalianum Wight.

Description

technical field [0001] The invention belongs to the technical field of camphorberry cultivation, in particular to a method for rooting, transplanting and hardening of camphorberry. Background technique [0002] Plant tissue culture technology is a technique for culturing isolated plant organs, tissues, cells and protoplasts with appropriate medium under sterile and artificially controlled environmental conditions to regenerate them into cells or complete plants. Its main function is to realize the rapid proliferation of materials in a geometric exponential manner. Generally, it can be divided into four stages: establishment of sterile system, multiplication culture, rooting culture, seedling hardening and transplanting. Only when the technical problems in each stage are solved and then organically combined can the rapid propagation of tissue culture seedlings be realized. At present, the plant tissue culture research of Vaccinium camphora leaves mainly stays in the stages o...

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Problems solved by technology

[0007] To sum up, the problems existing in the prior art are: the quality of rooted seedlings is poor, and the rooting rate needs to be improved.

[0008] Existing Vaccinium camphoria rooting and transplanting seedling hardening methods are not analyzed from the seedling hardening technology of test tube seedlings, and are not carried out from the comparison of rooting hormone types and dosage, the screening of basic rooting medium, and the screening of hardening substrates. analysis to improve the rooting rate of the proliferating seedlings and the survival rate of hardened seedlings

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