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Molecular marking method for improving content of soybean protein and marking combination thereof

A technology of soybean protein and molecular markers, which is applied in the direction of biochemical equipment and methods, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problems of inability to select breeding, etc., so as to reduce the selection workload, shorten the breeding period, and the physical interval short effect

Inactive Publication Date: 2018-06-15
CROP RES INST SHANDONG ACAD OF AGRI SCI
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Problems solved by technology

However, soybean protein content is a quantitative trait controlled by multiple genes, and protein-related QTLs are material-specific and cannot be used in molecular marker-assisted selection breeding for specific breeding populations.

Method used

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  • Molecular marking method for improving content of soybean protein and marking combination thereof
  • Molecular marking method for improving content of soybean protein and marking combination thereof
  • Molecular marking method for improving content of soybean protein and marking combination thereof

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Embodiment 1

[0021] Embodiment 1, a kind of molecular marking method that improves soybean protein content comprises the following steps:

[0022] 1) The distribution of protein content of soybean RIL population: Huapidou and Qihuang 26 high-generation RIL populations were planted for two consecutive years, and the protein content was counted. It was found that the protein content of the RIL population basically showed a continuous distribution; Figure 1-2 shown.

[0023] 2) QTL mapping of soybean protein content: The high-density genetic map constructed based on SLAF-seq technology was used to map the QTL of soybean RIL population protein content, and a total of 8 soybean protein content-related QTLs were obtained by using complete interval mapping (ICIM) site ( image 3 ), located on chromosomes 1, 3, 4, 5, 8, 10, 15 and 16 (Table 1), explaining 77.14% of the phenotypic variation. Such as image 3 shown.

[0024] Table 1 QTL loci related to soybean protein content

[0025]

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Abstract

The invention relates to a molecular marking method for improving the content of soybean protein. The molecular marking method comprises the following steps of: 1) distribution of the protein contentof RIL (Recombinant Inbred Line) populations of the soybean: carrying out continuous planting for two years on the advanced-generation RIL populations of smooth-skin soybeans and Qihuang 26, and calculating the protein content thereof; 2) QTL (Quantitative Trait Loci) location of the content of the soybean protein: utilizing a high-density genetic map constructed by an SLAF-seq technology to carryout QTL location on the protein content of the RIL populations of the soybean, and obtaining 8 QTL site associated with the protein content of the soybean. The molecular marking method has the beneficial effects that the marking combination provided by the invention is utilized for carrying out low-generation marking selection on breeding materials, so that the benefit for aggregating excellent genes of the protein in the breeding materials at low generations is achieved, the workload of advanced-generation breeding selection is reduced, the breeding scale is shrunk, and the breeding time isshortened.

Description

technical field [0001] The invention relates to the technical field of molecular marker-assisted breeding, in particular to a molecular marker method and marker combination for increasing soybean protein content. Background technique [0002] Soybean is an important grain, oil and feed crop, and has an important strategic position in the development of the national economy. Soybean is rich in protein, and its content is more than twice that of wheat, rice and other cereal crops, usually between 40% and 50%. The essential amino acid content in protein is sufficient and the proportion is coordinated, which is very close to the proportion required by human physiology. , can effectively replace the nutrients of animal protein. At the same time, soybean protein has good functional properties and can be used in a variety of food systems to improve product quality. [0003] At present, there are 221 QTLs related to soybean protein included in the SoyBase database. However, soybe...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 张彦威李伟林延慧张礼凤王彩洁徐冉
Owner CROP RES INST SHANDONG ACAD OF AGRI SCI
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