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Method for breeding all sterile line with genetic stability of rape recessive epistasis genic male sterile line by means of molecular marker

A molecular marker, genetically stable technology, applied in the fields of botanical equipment and methods, biochemical equipment and methods, and microbial determination/inspection, etc., can solve the complex genetic separation of hybrid offspring, limit the speed and advantages of the application and promotion of sterile systems Attenuation and other problems to achieve the effect of reducing the workload of breeding, improving the accuracy and success rate of breeding, and avoiding the decline of the advantage of poor uniformity

Active Publication Date: 2013-04-17
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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Problems solved by technology

However, there are two difficulties found in the breeding application that limit the application and promotion speed of the sterile system
One is that the genetic separation of hybrid offspring brought about by the multi-gene control system is complicated, and the improvement of two-type lines and temporary insurance lines is time-consuming and labor-intensive and inefficient; The species is a three-cross species, which will cause attenuation of dominance and decrease in purity to a certain extent, which may become a bottleneck problem for the large-scale and large-area application of the sterile system

Method used

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  • Method for breeding all sterile line with genetic stability of rape recessive epistasis genic male sterile line by means of molecular marker

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Embodiment Construction

[0022] Such as figure 1 The shown method for breeding the recessive epistatic genic male sterile line of rapeseed genetically stable total sterile line with the help of molecular markers, the technical scheme adopted by the method includes the following steps:

[0023] 1. Confirmation of the alleles of the recessive epistatic suppressor gene locus in the variety to be transformed: to ensure correctness, the variety to be transformed is firstly crossed with the homozygous sterile line in the stable two-type line and the temporary insurance line (as the male parent), F1 Self-fertilization, from the F2 generation to determine whether the variety to be transformed is MS 3 MS 3 RfRf (Sterile strains were isolated in the F2 generation of crossing with Lingbao line, sterile / fertile: 3 / 13) or MS 3 MS 3 The genotype of rfrf (no sterile strain segregation in F2 generation crossed with Temporary line).

[0024] 2. If the breed to be bred is MS 3 MS 3 RfRf genotype, proceed as follo...

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Abstract

The invention, belonging to the field of molecule breeding of rape, relates to a method for breeding an all sterile line with genetic stability of a rape recessive epistasis genic male sterile line by means of molecular marker. According to the invention, by the close link of molecular marker and fertility genes, the genotype of molecular mark is tracked and selected to select a program of the genotypes of a two-type line and a temporary maintainer line, so that the breeding strategy of the sterile line controlled by polygenes is improved from a process of complete phenotype identification toblanket search of genotype identification to a high efficiency breeding process of well-targeted marker genotype selection first to actual phenotype identification finally, by backcrossing and selfing multi-generation and field phenotype identification, the homozygous two type line and temporary maintainer line with same genetic background and uniform growth in fields are obtained, and then all sterile line seeds with genetic stability are produced for largely plantation. Therefore, the current situation that seeds for largely plantation of the sterile line are actual three-way cross hybrids is changed, and the problems of bad uniformity and declined advantages caused by the current situation are avoided.

Description

technical field [0001] The invention belongs to the field of molecular breeding of rapeseed, and in particular relates to a method for breeding a recessive epistasis nuclear sterile system genetically stable total sterile line of rapeseed by means of molecular markers. Background technique [0002] Male sterility is an important way to utilize heterosis in Brassica napus. The male sterile system involved in the present invention is a nuclear sterile system controlled by the interaction of a recessive sterile gene and a recessive epistatic suppressor gene (Chen Fengxiang et al., Acta Crops, 1998, 24(4): 431-438) , the sterile system has the characteristics of high sterility, stable sterility, fully open petals, high seed yield, and common rapeseed varieties are all natural restorer lines. Chen Fengxiang et al. proposed a "three-line" utilization model in which a total sterile line was obtained by crossing a homozygous "two-type line" and a "temporary insurance line" and then...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68A01H1/04A01H1/02C12N15/11
Inventor 赵坚义倪西源徐小栋黄吉祥陈飞
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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