The invention, belonging to the field of molecule breeding of rape, relates to a method for breeding an all sterile line with
genetic stability of a rape recessive epistasis genic male sterile line by means of
molecular marker. According to the invention, by the close link of
molecular marker and
fertility genes, the
genotype of molecular mark is tracked and selected to select a program of the genotypes of a two-type line and a temporary
maintainer line, so that the breeding strategy of the sterile line controlled by polygenes is improved from a process of complete
phenotype identification toblanket search of
genotype identification to a high efficiency breeding process of well-targeted marker
genotype selection first to actual
phenotype identification finally, by
backcrossing and
selfing multi-generation and field
phenotype identification, the homozygous two type line and temporary
maintainer line with same genetic background and uniform growth in fields are obtained, and then all sterile line seeds with
genetic stability are produced for largely plantation. Therefore, the current situation that seeds for largely plantation of the sterile line are actual three-way cross hybrids is changed, and the problems of bad uniformity and declined advantages caused by the current situation are avoided.