Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of protein page gel electrophoresis rapid staining kit and staining method

A rapid staining and gel electrophoresis technology, applied in the field of protein analysis and detection, can solve the problem of long staining time, and achieve the effect of short experiment time, time saving and high contrast.

Active Publication Date: 2021-02-19
上海中科新生命生物科技有限公司
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the disadvantage is that the dyeing time is longer, it needs at least one hour of dyeing, usually overnight treatment, and then decolorization three times, the total time-consuming needs tens of hours

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of protein page gel electrophoresis rapid staining kit and staining method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Protein PAGE gel electrophoresis rapid staining kit.

[0023] A fast staining kit for protein PAGE gel electrophoresis, which includes a series of reagents for HSC dyes, two series of reagents for HSC dyes and water; the series of reagents for HSC dyes includes absolute alcohol and phosphoric acid; the second series of HSC dyes Reagents include Coomassie brilliant blue G-250, ammonium sulfate, phosphoric acid, methanol, aluminum sulfate.

Embodiment 2

[0024] Embodiment two: a kind of fast staining method using above-mentioned kit

[0025] A rapid staining method using the above kit, which includes the following detailed steps:

[0026] Step S10 to make HSC dye 1, measure 500mL of absolute ethanol and 80mL of phosphoric acid, mix them evenly, use deionized water to make up to 1000ml, and store them in a brown bottle for later use;

[0027] Step S20 to make HSC dye 2, weigh 50g of aluminum sulfate, 100g of ammonium sulfate, put them into a 1000ml beaker, mix them, then pour 100mL of phosphoric acid and 100ml of ultrapure water into the beaker, put #2 stirring bar in the beaker, and transfer Put it on a heating stirrer, and stir for 1.5 hours at a temperature of 40°C and a speed of 400r / min until completely dissolved. After complete dissolution, add 1.2g Coomassie Brilliant Blue G-250 and 400ml ultrapure water to the beaker, stir at 600r / min for 30min at room temperature; add 200ml methanol after stirring, seal with parafilm ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a protein PAGE gel electrophoresis rapid-staining kit and a staining method thereof, and belongs to the technical field of protein analysis and detection. The kit comprises HSCdye 1 series reagents, HSC dye 2 series reagents and water; the HSC dye 1 series reagents include anhydrous ethanol and phosphoric acid; and the HSC dye 2 series reagents include Coomassie brilliant blue G-250, ammonium sulfate, phosphoric acid, methanol and aluminum sulfate, and staining using an HSC dye 2 is performed after fixing using an HSC dye 1. The kit and the method have the advantages ofshort experimental time, high detection sensitivity and good mass spectrometry compatibility.

Description

technical field [0001] The invention belongs to the technical field of protein analysis and detection, and in particular relates to a protein PAGE gel electrophoresis rapid staining kit and also relates to a protein PAGE gel electrophoresis rapid staining method. Background technique [0002] Electrophoresis is a main method or technique for separating proteins and other substances such as nucleic acids, purines, pyrimidines, some organic compounds and even inorganic ions. The common staining method for routine detection and quantification of proteins separated by electrophoresis is Coomassie Brilliant Blue R250 staining. [0003] The traditional dyeing method is to dye the glue with Coomassie Brilliant Blue R250, methanol, acetic acid, and aqueous solution. Coomassie Brilliant Blue R250 staining can detect 30-100ng of protein, the sensitivity is much lower than silver staining and fluorescent staining, and it is easy to operate, requires less reagents, has no toxicity, the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/31C07K1/26
CPCC07K1/26G01N1/31
Inventor 王东茂陈薇阮宏强
Owner 上海中科新生命生物科技有限公司