Digital microscopic imaging system and method

A technology of digital microscopy and imaging system, applied in the field of digital microscopy, can solve the problems of loss of object information, affecting the quality of reproduced images, poor accuracy, etc., to achieve the effect of improving accuracy

Active Publication Date: 2018-06-15
河北荣众信息科技有限公司
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Problems solved by technology

However, when the beam combiner is rotated, if the rotation angle is small, the off-axis holography in which the reconstructed image is completely separated may not be obtained, which affects the quality of the reconstructed image; if the rotation angle is large, part of the object light wave information may not be referenced. Recorded, thereby losing object information
In the prior art, the adjustment of the rotation angle of the beam combiner is done manually. This adjustment method has poor accuracy and affects the reproduction quality of the digital holographic image.

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  • Digital microscopic imaging system and method

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Embodiment Construction

[0011] Off-axis digital holography is mainly divided into off-axis Fresnel digital holography and off-axis lensless Fourier transform digital holography. This application takes the recording of off-axis Fresnel digital holography as an example to introduce the recording of off-axis digital holograms. And how to precisely adjust the angle between the object light wave and the reference light wave during the recording process, so as to obtain high-quality reproduced images.

[0012] exist figure 2 In the schematic diagram of the off-axis Fresnel digital holographic recording optical path shown, the angles between the plane reference light wave of unit amplitude and the yoz plane and the xoz plane are respectively α and β, and according to the Fresnel diffraction formula, in the paraxial approximation Under the condition of ignoring the constant phase factor, the distributions of the reference light wave and the object light wave arriving at the CCD plane are respectively

[00...

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Abstract

The invention relates to a microscopic imaging system and method. The imaging system is characterized in that light emitted from a laser is divided into two beams of light waves, wherein one beam of light wave carries information of an object, and the other beam of light wave is subjected to beam expansion and collimation to obtain a planar optical wave; the two beams of light waves interfere on aCCD to form an interference pattern; and the interference pattern is reproduced through a computer to obtain a three-dimensional microscopic image of the object. The included angle of the two beams of light waves is controlled automatically, so that an original image and a zero-order interference image in the reproduced image are allowed to be just separated, and the highest-resolution original image can be obtained.

Description

technical field [0001] The application relates to a digital microscope, which belongs to the field of optical microscopic imaging. Background technique [0002] The usual microscope is mainly obtained through the lens group, the microscopic image obtained in this way cannot be recorded and saved, and the details of the image will not be remembered very quickly after observation. With the continuous development of semiconductor technology and laser technology, a digital microscope has appeared at this stage, which can obtain the phase image of the imaged object while obtaining the intensity image of the imaged object, or it can obtain the three-dimensional image of the imaged object. And the obtained three-dimensional image can be permanently preserved. [0003] Digital microscope technology uses photocoupler devices (CCD or CMOS) to record interference fringes, and then sends them to the computer to reconstruct the intensity and phase distribution of the object through algo...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G02B21/36G03H1/04
CPCG02B21/361G02B21/362G03H1/0443G03H2001/005G03H2001/0445G03H2001/045
Inventor 王华英朱巧芬熊南燕张步勤王鹏江夏男董昭张雷
Owner 河北荣众信息科技有限公司
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